Elucidation of Antibacterial Compounds from Inflorescences of Banana (Musa balbisiana cv. Saba) Using Liquid Chromatography-Tandem Mass Spectrometry

Author(s): Hoe S. Tin, Birdie S. Padam, Fook Y. Chye*

Journal Name: Current Nutrition & Food Science

Volume 16 , Issue 7 , 2020


Become EABM
Become Reviewer
Call for Editor

Graphical Abstract:


Abstract:

Background: Banana by-products are undervalued and their potential remains untapped. They are often composted after the fruits are harvested, reducing the cause of environmental pollutions due to open burning.

Objectives: The study aims to identify the bioactive compounds in banana [Musa acuminate x balbisiana Colla cv. Saba (Musaceae)] inflorescence buds that are responsible for the antibacterial activity on selected foodborne pathogens.

Methods: Dried inflorescence buds were extracted using methanol and subsequently partitioned into chloroform, ethyl acetate, butanol and deionized water. Further isolation of bioactive components was based on a bioassay-guided fractionation and the inhibitory activity at various concentrations against selected foodborne pathogens was determined. The compounds were identified using highperformance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-MS/MS).

Results: The SPE-fraction 3 (BWF-3) purified from the methanolic water partition of banana inflorescence showed the most prominent inhibition against Staphylococcus aureus with minimum inhibitory concentration at 12.0 μg/ml. The BWF-3 was later identified as proanthocyanidins with epigallocatechin as the main extension unit. Additionally, the survival of Listeria monocytogenes increased with the fortification of ferum (II) and (III) at a concentration as low as 1 mM but not for the calcium, magnesium, manganese and glucose.

Conclusion: The methanolic partition of banana inflorescence buds could be a potential source of natural antibacterial for food and pharmaceutical applications.

Keywords: Banana by-product, epigallocatechin, foodborne pathogens, inflorescence buds, Musa acuminate, proanthocyanidin.

[1]
Bakal SN, Bereswill S, Heimesaat MM. Finding novel antibiotic substances from medicinal plants - Antimicrobial properties of Nigella sativa directed against multidrug resistant bacteria. Eur J Microbiol Immunol (Bp) 2017; 7(1): 92-8.
[http://dx.doi.org/10.1556/1886.2017.00001] [PMID: 28386474]
[2]
Caniça M, Manageiro V, Abriouel H, Moran-Gilad J, Franz CMAP. Antibiotic resistance in foodborne bacteria. Trends Food Sci Technol 2018; 84: 41-4.
[http://dx.doi.org/10.1016/j.tifs.2018.08.001]
[3]
Patridge E, Gareiss P, Kinch MS, Hoyer D. An analysis of FDA-approved drugs: natural products and their derivatives. Drug Discov Today 2016; 21(2): 204-7.
[http://dx.doi.org/10.1016/j.drudis.2015.01.009] [PMID: 25617672]
[4]
Jain N, Bhatia A, Pathak H. Emission of air pollutants from crop residue burning in India. Aerosol Air Qual Res 2014; 14: 422-30.
[http://dx.doi.org/10.4209/aaqr.2013.01.0031]
[5]
Oliveira DA, Salvador AA, Smânia A Jr, Smânia EF, Maraschin M, Ferreira SR. Antimicrobial activity and composition profile of grape (Vitis vinifera) pomace extracts obtained by supercritical fluids. J Biotechnol 2013; 164(3): 423-32.
[http://dx.doi.org/10.1016/j.jbiotec.2012.09.014] [PMID: 23036924]
[6]
FAO. FAOSTAT. Food and Agriculture Organization of the United Nations, Rome, Italy [Online] 2017 [Cited 2019]. Available at 2017.http://www.fao.org/faostat/en/?#data/QC
[7]
De Oliveira Maia BG, De Oliveira APN, De Oliveira TMN, Marangoni C, Souza O, Sellin N. Characterization and production of banana crop and rice processing waste briquettes. Environ Prog Sustain Energy 2017; 37(4): 1266-73.
[http://dx.doi.org/10.1002/ep.12798]
[8]
Maran JP, Priya B, Al-Dhabi NA, Ponmurugan K, Moorthy IG, Sivarajasekar N. Ultrasound assisted citric acid mediated pectin extraction from industrial waste of Musa balbisiana. Ultrason Sonochem 2017; 35((Pt A)): 204-9.
[http://dx.doi.org/10.1016/j.ultsonch.2016.09.019] [PMID: 27707645]
[9]
Guerrero AB, Ballesteros I, Ballesteros M. The potential of agricultural banana waste for bioethanol production. Fuel 2018; 213: 176-85.
[http://dx.doi.org/10.1016/j.fuel.2017.10.105]
[10]
Wobiwo FA, Emaga TH, Fokou E, et al. Comparative biochemical methane potential of some varieties of residual banana biomass and renewable energy potential. Biomass Convers Bior 2016; 7(2): 167-77.
[http://dx.doi.org/10.1007/s13399-016-0222-x]
[11]
Wang H, Liu T, Song L, Huang D. Profiles and α-amylase inhibition activity of proanthocyanidins in unripe Manilkara zapota (chiku). J Agric Food Chem 2012; 60(12): 3098-104.
[http://dx.doi.org/10.1021/jf204715q] [PMID: 22394060]
[12]
Dai Y, Sun Q, Wang W, et al. Utilizations of agricultural waste as adsorbent for the removal of contaminants: A review. Chemosphere 2018; 211: 235-53.
[http://dx.doi.org/10.1016/j.chemosphere.2018.06.179] [PMID: 30077103]
[13]
Nik Yusuf NAA, Rosly ES, Mohamed M, et al. Waste banana peel and its potentialization in agricultural applications: Morphology overview. Materi Sci Forum. 2016; 840: 394-8.www.scientific.net/msf.840.3
[14]
Pramote B, Waranuch N, Kritsunankul O. Simultaneous determination of gallic acid and catechins in banana peel extract by reversed-phase high performance liquid chromatography. Naresuan Univ J Sci Technol 2018; 26: 189-200.
[15]
Rebello LPG, Ramos AM, Pertuzatti PB, Barcia MT, Castillo-Muñoz N, Hermosín-Gutiérrez I. Flour of banana (Musa AAA) peel as a source of antioxidant phenolic compounds. Food Res Int 2014; 55: 397-403.
[http://dx.doi.org/10.1016/j.foodres.2013.11.039]
[16]
Kandasamy S, Ramu S, Aradhya SM. In vitro functional properties of crude extracts and isolated compounds from banana pseudostem and rhizome. J Sci Food Agric 2016; 96(4): 1347-55.
[http://dx.doi.org/10.1002/jsfa.7229] [PMID: 25914102]
[17]
Valgas C, De Souza SM, Smania EFA, Smania A. Screening methods to determine antibacterial activity of natural products. Braz J Microbiol 2007; 38: 369-80.
[http://dx.doi.org/10.1590/S1517-83822007000200034]
[18]
Wayne PA, Ed. CLSI. Methods for dilution antimicrobial susceptibility test for bacteria that grow aerobically: Approved Standards- Tenth Edition. CLSI document M07-A10Clinical and Laboratory Standards Institute. 2015.
[19]
Pacheco-Ordaz R, Wall-Medrano A, Goñi MG, Ramos-Clamont-Montfort G, Ayala-Zavala JF, González-Aguilar GA. Effect of phenolic compounds on the growth of selected probiotic and pathogenic bacteria. Lett Appl Microbiol 2018; 66(1): 25-31.
[http://dx.doi.org/10.1111/lam.12814] [PMID: 29063625]
[20]
Engels C, Schieber A, Gänzle MG. Inhibitory spectra and modes of antimicrobial action of gallotannins from mango kernels (Mangifera indica L.). Appl Environ Microbiol 2011; 77(7): 2215-23.
[http://dx.doi.org/10.1128/AEM.02521-10] [PMID: 21317249]
[21]
Xu Y, Burton S, Kim C, Sismour E. Phenolic compounds, antioxidant, and antibacterial properties of pomace extracts from four Virginia-grown grape varieties. Food Sci Nutr 2015; 4(1): 125-33.
[http://dx.doi.org/10.1002/fsn3.264] [PMID: 26788319]
[22]
Abedini A, Roumy V, Mahieux S, et al. Antimicrobial activity of selected Iranian medicinal plants against a broad spectrum of pathogenic and drug multiresistant micro-organisms. Lett Appl Microbiol 2014; 59(4): 412-21.
[http://dx.doi.org/10.1111/lam.12294] [PMID: 24888993]
[23]
Navarro-Hoyos M, Lebrón-Aguilar R, Quintanilla-López JE, et al. Proanthocyanidin characterization and bioactivity of extracts from different parts of Uncaria tomentosa L. (Cat’s claw). Antioxidants 2017; 6(1): 12-30.
[http://dx.doi.org/10.3390/antiox6010012] [PMID: 28165396]
[24]
Cho JY, Sohn MJ, Lee J, Kim WG. Isolation and identification of pentagalloylglucose with broad-spectrum antibacterial activity from Rhus trichocarpa Miquel. Food Chem 2010; 123: 501-6.
[http://dx.doi.org/10.1016/j.foodchem.2010.04.072]
[25]
Papuc C, Goran GV, Predescu CN, Nicorescu V, Stefan G. Plant polyphenols as antioxidant and antibacterial agents for shelf-life extension of meat and meat products: classification, structures, sources, and action mechanisms. Compr Rev Food Sci Food Saf 2017; 16(6): 1243-68.
[http://dx.doi.org/10.1111/1541-4337.12298]
[26]
Perumal S, Mahmud R, Ismail S. Mechanism of action of isolated caffeic acid and epicatechin 3-gallate from Euphorbia hirta against Pseudomonas aeruginosa. Pharmacogn Mag 2017; 13(2)(Suppl. 2): S311-5.
[http://dx.doi.org/10.4103/pm.pm_309_15] [PMID: 28808398]
[27]
Wu Y, Bai J, Zhong K, et al. Antibacterial activity and membrane-disruptive mechanism of 3-p-trans-coumaroyl-2-hydroxyquinic acid, a novel phenolic compound from pine needles of Cedrus deodara, against Staphylococcus aureus. Molecules 2016; 21(8): 1084-96.
[http://dx.doi.org/10.3390/molecules21081084] [PMID: 27548123]
[28]
Campone L, Piccinelli AL, Pagano I, et al. Determination of phenolic compounds in honey using dispersive liquid-liquid microextraction. J Chromatogr A 2014; 1334: 9-15.
[http://dx.doi.org/10.1016/j.chroma.2014.01.081] [PMID: 24565235]
[29]
Sergiel I, Pohl P, Biesaga M. Characterisation of honeys according to their content of phenolic compounds using high performance liquid chromatography/tandem mass spectrometry. Food Chem 2014; 145: 404-8.
[http://dx.doi.org/10.1016/j.foodchem.2013.08.068] [PMID: 24128495]
[30]
Silva S, Costa EM, Mendes M, Morais RM, Calhau C, Pintado MM. Antimicrobial, antiadhesive and antibiofilm activity of an ethanolic, anthocyanin-rich blueberry extract purified by solid phase extraction. J Appl Microbiol 2016; 121(3): 693-703.
[http://dx.doi.org/10.1111/jam.13215] [PMID: 27349348]
[31]
Zhang L, Tai Y, Wang Y, et al. The proposed biosynthesis of procyanidins by the comparative chemical analysis of five Camellia species using LC-MS. Sci Rep 2017; 7(1): 46131.
[http://dx.doi.org/10.1038/srep46131] [PMID: 28383067]
[32]
Wang J, Hu Y. Novel particleboard composites made from coir fiber and waste banana stem fiber. Waste Biomass Valoriz 2016; 7(6): 1447-58.
[http://dx.doi.org/10.1007/s12649-016-9523-3]
[33]
Deng Y, Yang G, Yue J, et al. Influences of ripening stages and extracting solvents on the polyphenolic compounds, antimicrobial and antioxidant activities of blueberry leaf extracts. Food Control 2014; 38: 184-91.
[http://dx.doi.org/10.1016/j.foodcont.2013.10.023]
[34]
Reygaert WC. The antimicrobial possibilities of green tea. Front Microbiol 2014; 5: 434-42.
[http://dx.doi.org/10.3389/fmicb.2014.00434] [PMID: 25191312]
[35]
Jeon J, Kim JH, Lee CK, Oh CH, Song HJ. The antimicrobial activity of (-)-epigallocatehin-3-gallate and green tea extracts against Pseudomonas aeruginosa and Escherichia coli isolated from skin wounds. Ann Dermatol 2014; 26(5): 564-9.
[http://dx.doi.org/10.5021/ad.2014.26.5.564] [PMID: 25324647]
[36]
Maria JKM, Mandal AKA, Rajesh J, Sampath N. Antioxidant and antimicrobial activity of individual catechin molecules: A comparative study between gallated and epimerized catechin molecules. Res J Biotechnol 2012; 7: 5-9.
[37]
Sadiq MB, Hanpithakpong W, Tarning J, Anal AK. Screening of phytochemicals and in vitro evaluation of antibacterial and antioxidant activities of leaves, pods and bark extracts of Acacia nilotica (L.). Del Ind Crop Prod 2015; 77: 873-82.
[http://dx.doi.org/10.1016/j.indcrop.2015.09.067]


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 16
ISSUE: 7
Year: 2020
Page: [1130 - 1140]
Pages: 11
DOI: 10.2174/1573401316666200120125601
Price: $65

Article Metrics

PDF: 26
HTML: 2