Background: The present study assessed the transdermal potential of transferosomes
loaded with allopurinol for the treatment of gout.
Methods: Transferosomes of allopurinol were composed of different ratios of tween-80, soya
lecithin and solvent using a thin-film hydration method. Transferosomes were characterized for
Scanning Electron Microscopy (SEM), zeta potential, % entrapment efficiency (%EE), Fourier
Transform Infrared Spectroscopy (FTIR), in-vitro drug release and kinetics as well as stability.
Then, optimized formulation was incorporated in gel and evaluated for viscosity, pH, extrudability,
homogeneity, skin irritation study, spreadability, ex vivo skin permeation study, flux, and stability.
Results: SEM studies suggested that vesicles were spherical and zeta potential were in the range of
-11.4 mV to -29.6 mV and %EE was 52.4- 83.87%. FTIR study revealed that there was no interaction
between allopurinol and excipients during the preparation of transferosomes. The cumulative
percentage of drug release from various transferosomes was ranged from 51.87 to 81.87%. A transferosomal
gel of F8 formulation was prepared using dispersion method reported pseudoplastic
rheological behavior, optimum pH, spreadability and maximum drug permeation i.e. 79.84% with
flux 13.06 g/cm2/hr, followed zero-order release kinetics. Irritation and in-vivo studies of optimized
transferosomal gel G8 on rabbits revealed better results than the standard allopurinol.
Conclusion: This research suggested that allopurinol loaded transferosomal gel can be potentially
used as a transdermal drug delivery system for the treatment of gout.