Effects of Sulfur Starvation on Growth Rates, Biomass and Lipid Contents in the Green Microalga Scenedesmus obliquus

Author(s): Mohammad H. Morowvat*, Younes Ghasemi

Journal Name: Recent Patents on Biotechnology

Volume 14 , Issue 2 , 2020

Become EABM
Become Reviewer

Graphical Abstract:


Abstract:

Background: Scenedesmus obliquus, a green unicellular chlorophycean microalga, is well-established as a lipid and biomass production platform. The nutrient starvation strategy is considered as a robust platform for lipid production from different microalgal strains.

Objective: The study aimed to analyse the influences of sulfur starvation on the growth rates, and also biomass and lipid production and composition in a naturally isolated strain of S. obliquus.

Methods: The BG-11 culture medium was utilized for preservation and microalgal growth. To monitor the cell growth rates, two different methods, including direct cell counting and also dry cell weight measurement were used. The study was conducted in 28 days composed of two distinct growth modes as 10 days of sulfur-rich and 18 days of sulfur starved media.

Results: The studied S. obliquus strain displayed higher lipid and carbohydrate production levels (34.68% and 34.02%) in sulfur starved medium compared with the sulfur-rich medium (25.84% and 29.08%). Nevertheless, a noticeable reduction (51.36%) in biomass contents and also in cell growth rates (63.36%) was observed during sulfur starvation. The investigated strain was composed of some important fatty acids with potential applications as food, feed and biodiesel.

Conclusion: The observed results implied the possibility of the sulfur starvation strategy to increase lipid production in S. obliquus strain. Besides, the available data from recently published patents reveals the promising potential of the identified lipids from S. obliquus in this study for bioenergy production and other biotechnological purposes.

Keywords: Biomass composition, fatty acid profile, lipid content, Scenedesmus obliquus, sulfur starvation, microalgal strains.

[1]
Mandotra SK, Kumar P, Suseela MR, Ramteke PW. Fresh water green microalga Scenedesmus abundans: A potential feedstock for high quality biodiesel production. Bioresour Technol 2014; 156: 42-7.
[http://dx.doi.org/10.1016/j.biortech.2013.12.127] [PMID: 24486936]
[2]
Álvarez-Díaz PD, Ruiz J, Arbib Z, Barragán J, Garrido-Pérez MC, Perales JA. Wastewater treatment and biodiesel production by Scenedesmus obliquus in a two-stage cultivation process. Bioresour Technol 2015; 181: 90-6.
[http://dx.doi.org/10.1016/j.biortech.2015.01.018] [PMID: 25643954]
[3]
Benavente-Valdés JR, Aguilar C, Contreras-Esquivel JC, Méndez-Zavala A, Montañez J. Strategies to enhance the production of photosynthetic pigments and lipids in chlorophycae species. Biotechnol Rep (Amst) 2016; 10: 117-25.
[http://dx.doi.org/10.1016/j.btre.2016.04.001] [PMID: 28352532]
[4]
Zhu LD, Li ZH, Hiltunen E. Strategies for lipid production improvement in microalgae as a biodiesel feedstock. BioMed Res Int 2016; 20168792548
[http://dx.doi.org/10.1155/2016/8792548] [PMID: 27725942]
[5]
Mandotra SK, Kumar P, Suseela MR, Nayaka S, Ramteke PW. Evaluation of fatty acid profile and biodiesel properties of microalga Scenedesmus abundans under the influence of phosphorus, pH and light intensities. Bioresour Technol 2016; 201: 222-9.
[http://dx.doi.org/10.1016/j.biortech.2015.11.042] [PMID: 26675046]
[6]
Anand J, Arumugam M. Enhanced lipid accumulation and biomass yield of Scenedesmus quadricauda under nitrogen starved condition. Bioresour Technol 2015; 188: 190-4.
[http://dx.doi.org/10.1016/j.biortech.2014.12.097] [PMID: 25641714]
[7]
Chu FF, Chu PN, Shen XF, Lam PKS, Zeng RJ. Effect of phosphorus on biodiesel production from Scenedesmus obliquus under nitrogen-deficiency stress. Bioresour Technol 2014; 152: 241-6.
[http://dx.doi.org/10.1016/j.biortech.2013.11.013] [PMID: 24292204]
[8]
Praba T, Ajan C, Citarasu T, Selvaraj T, Dhas AS, Gopal P, et al. Effect of different culture media for the growth and oil yield in selected marine microalgae. J Aquacult Trop 2016; 31(3/4): 165.
[9]
Solovchenko AE. Physiological role of neutral lipid accumulation in eukaryotic microalgae under stresses. Russ J Plant Physiol 2012; 59(2): 167-76.
[http://dx.doi.org/10.1134/S1021443712020161]
[10]
Marieschi M, Gorbi G, Zanni C, Sardella A, Torelli A. Increase of chromium tolerance in Scenedesmus acutus after sulfur starvation: Chromium uptake and compartmentalization in two strains with different sensitivities to Cr(VI). Aquat Toxicol 2015; 167: 124-33.
[http://dx.doi.org/10.1016/j.aquatox.2015.08.001] [PMID: 26281774]
[11]
Masuko T, Minami A, Iwasaki N, Majima T, Nishimura S, Lee YC. Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Anal Biochem 2005; 339(1): 69-72.
[http://dx.doi.org/10.1016/j.ab.2004.12.001] [PMID: 15766712]
[12]
Vendruscolo RG, Fagundes MB, Maroneze MM, et al. Scenedesmus obliquus metabolomics: effect of photoperiods and cell growth phases. Bioprocess Biosyst Eng 2019; 42(5): 727-39.
[http://dx.doi.org/10.1007/s00449-019-02076-y] [PMID: 30671626]
[13]
Ren H-Y, Liu B-F, Ma C, Zhao L, Ren N-Q. A new lipid-rich microalga Scenedesmus sp. strain R-16 isolated using Nile red staining: effects of carbon and nitrogen sources and initial pH on the biomass and lipid production. Biotechnol Biofuels 2013; 6(1): 143.
[http://dx.doi.org/10.1186/1754-6834-6-143] [PMID: 24093331]
[14]
Goswami RCD, Kalita M. Scenedesmus dimorphus and Scenedesmus quadricauda: two potent indigenous microalgae strains for biomass production and CO2 mitigation-a study on their growth behavior and lipid productivity under different concentration of urea as nitrogen source. J Algal Biomass Util 2011; 2(4): 2-4.
[15]
Yin-Hu W, Yin Y, Xin L, Hong-Ying H, Zhen-Feng S. Biomass production of a Scenedesmus sp. under phosphorous-starvation cultivation condition. Bioresour Technol 2012; 112: 193-8.
[http://dx.doi.org/10.1016/j.biortech.2012.02.037] [PMID: 22424927]
[16]
Gorbi G, Zanni C, Corradi MG. Sulfur starvation and chromium tolerance in Scenedesmus acutus: a possible link between metal tolerance and the regulation of sulfur uptake/assimilation processes. Aquat Toxicol 2007; 84(4): 457-64.
[http://dx.doi.org/10.1016/j.aquatox.2007.07.006] [PMID: 17727973]
[17]
Saito K. Regulation of sulfate transport and synthesis of sulfur-containing amino acids. Curr Opin Plant Biol 2000; 3(3): 188-95.
[http://dx.doi.org/10.1016/S1369-5266(00)00063-7] [PMID: 10837270]
[18]
Gigolashvili T, Kopriva S. Transporters in plant sulfur metabolism. Front Plant Sci 2014; 5: 442.
[http://dx.doi.org/10.3389/fpls.2014.00442] [PMID: 25250037]
[19]
Anjum NA, Gill R, Kaushik M, et al. ATP-sulfurylase, sulfur-compounds, and plant stress tolerance. Front Plant Sci 2015; 6: 210.
[http://dx.doi.org/10.3389/fpls.2015.00210] [PMID: 25904923]
[20]
Vega JM. Nitrogen and sulfur metabolism in microalae and plants: 50 years of research. Berlin, Heidelberg: Springer 2018.
[21]
Chen H, Zheng Y, Zhan J, He C, Wang Q. Comparative metabolic profiling of the lipid-producing green microalga Chlorella reveals that nitrogen and carbon metabolic pathways contribute to lipid metabolism. Biotechnol Biofuels 2017; 10: 153.
[http://dx.doi.org/10.1186/s13068-017-0839-4] [PMID: 28630648]
[22]
Yang D, Song D, Kind T, Ma Y, Hoefkens J, Fiehn O. Lipidomic analysis of Chlamydomonas reinhardtii under nitrogen and sulfur deprivation. PLoS One 2015; 10(9)e0137948
[http://dx.doi.org/10.1371/journal.pone.0137948] [PMID: 26375463]
[23]
Shaker S, Morowvat MH, Ghasemi Y. Effects of sulfur, iron and manganese starvation on growth, carotene production and lipid profile of Dunaliella salina. J Young Pharm 2017; 9(1): 43-6.
[http://dx.doi.org/10.5530/jyp.2017.9.9]
[24]
El-Sheekh M, Abomohra Ael-F, Hanelt D. Optimization of biomass and fatty acid productivity of Scenedesmus obliquus as a promising microalga for biodiesel production. World J Microbiol Biotechnol 2013; 29(5): 915-22.
[http://dx.doi.org/10.1007/s11274-012-1248-2] [PMID: 23269508]
[25]
Jenkins B, West JA, Koulman A. A review of odd-chain fatty acid metabolism and the role of pentadecanoic Acid (c15:0) and heptadecanoic Acid (c17:0) in health and disease. Molecules 2015; 20(2): 2425-44.
[http://dx.doi.org/10.3390/molecules20022425] [PMID: 25647578]
[26]
Shao Y, Fang H, Zhou H, Wang Q, Zhu Y, He Y. Detection and imaging of lipids of Scenedesmus obliquus based on confocal Raman microspectroscopy. Biotechnol Biofuels 2017; 10: 300.
[http://dx.doi.org/10.1186/s13068-017-0977-8] [PMID: 29255483]
[27]
Roe CR. Five and fifteen carbon fatty acids for treating metabolic disorders and as nutritional supplements. US8399515B2 2013.
[28]
Hee-moo O, Gug C, Tae KY, Sik KH, Yong A. Scenedesmus sp. M001 producing biodiesel, and method for producing biodiesel using the strain 2010. KR101424315B 2010.
[29]
Chinnasamy S, Bhatangar A, Hunt RW, Claxton R, Marlowe M, Das KC. Microalgae cultivation in a wastewater dominated by carpet mill effluents for biofuel applications. US20100267122A1 2010.
[30]
Kochabashi K, Papazis AI. Hydrogen production from chloro-algae (Scendesmus obliquus) via dichlorophenols biodegradation. GR1007207B 2009.
[31]
Xinqing Z, Suolian G, Fengwu B, Jiaxiu Z. Scenedesmus obliquus producing self-flocculation substance, and application of same in microalgae harvesting. CN102943046B 2012.
[32]
Yanling Y, Yujie F, Dawei Z, Chao Y. Scendesmus obliquus capable of synchronously processing municipal sewage and accumulating grease. CN103484374B 2013.
[33]
Hirabayashi S, Prilutsky A, Sadamatsu H. Method of culturing algae capable of producing phototrophic pigments, highly unsaturated faty acids, or polysaccharides at high concentraion. US6579714B 2000.


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 14
ISSUE: 2
Year: 2020
Published on: 11 May, 2020
Page: [145 - 153]
Pages: 9
DOI: 10.2174/1872208314666200109103059
Price: $65

Article Metrics

PDF: 10
HTML: 2