Background: For the first time, the investigation of six anti-inflammatory drugs and six antihistaminic drugs for inhibitory activities against alpha-amylase has been evaluated using a new inhibition detection method in order to find new treatments for some diseases caused by α-amylase.
Objective: The first part of this work was devoted to the evaluation of the inhibition activity of these drugs on salivary α-amylase in vitro. Then to study the nature of interactions and structure-activity relationship, using Autodockvina program for molecular docking.
Method: The evaluation of the inhibitory activity of our drugs is achieved using a new method that has proved its sensitivity, quickness and effectiveness.
Results: The results of this study show that the betamethasone and loratadine are potent α-amylase inhibitors with IC50 values 0.7mg/ml and 1.03 mg/ml, respectively compared to acarbose with IC50=5.6 µg/ml.
Conclusion: The results showed that the loratadine and the betamethasone have a strong potential to inhibit the alpha amylase.