Background: For the first time, the investigation of six anti-inflammatory drugs and six
antihistaminic drugs for inhibitory activities against alpha-amylase has been evaluated using a new
inhibition detection method in order to find new treatments for some diseases caused by α-amylase.
Objective: The first part of this work was devoted to the evaluation of the inhibition activity of these
drugs on salivary α-amylase in vitro. Then to study the nature of interactions and structure-activity
relationship, using the Autodockvina program for molecular docking.
Materials and Methods: The evaluation of the inhibitory activity of our drugs is achieved using a
new method that has proved its sensitivity, quickness, and effectiveness.
Results: The results of this study show that betamethasone and loratadine are potent α-amylase inhibitors
with IC50 values 0.7mg/ml and 1.03 mg/ml, respectively compared to acarbose with IC50=5.6
Conclusion: The results showed that the loratadine and the betamethasone have a strong potential to
inhibit the alpha-amylase.