New Inhibition Detection Method to Evaluate the Human Salivary Alphaamylase Activity of Some Drugs, Molecular Docking, and SAR Studies

Author(s): Nia Samira*, Benarous Khedidja, Lakaas Manel, Sadeki Israa, Yousfi Mohamed

Journal Name: Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry
Formerly Current Medicinal Chemistry - Anti-Inflammatory & Anti-Allergy Agents

Volume 20 , Issue 1 , 2021


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Graphical Abstract:


Abstract:

Background: For the first time, the investigation of six anti-inflammatory drugs and six antihistaminic drugs for inhibitory activities against alpha-amylase has been evaluated using a new inhibition detection method in order to find new treatments for some diseases caused by α-amylase.

Objective: The first part of this work was devoted to the evaluation of the inhibition activity of these drugs on salivary α-amylase in vitro. Then to study the nature of interactions and structure-activity relationship, using the Autodockvina program for molecular docking.

Materials and Methods: The evaluation of the inhibitory activity of our drugs is achieved using a new method that has proved its sensitivity, quickness, and effectiveness.

Results: The results of this study show that betamethasone and loratadine are potent α-amylase inhibitors with IC50 values 0.7mg/ml and 1.03 mg/ml, respectively compared to acarbose with IC50=5.6 μg/ml.

Conclusion: The results showed that the loratadine and the betamethasone have a strong potential to inhibit the alpha-amylase.

Keywords: Anti-inflammatory drugs, antihistaminic drugs, human salivary α-amylase, inhibition activity, molecular docking, betamethasone.

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Article Details

VOLUME: 20
ISSUE: 1
Year: 2021
Published on: 10 March, 2021
Page: [10 - 19]
Pages: 10
DOI: 10.2174/1871523019666200102111048
Price: $65

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