A Potent In Vitro α-Amylase Inhibitory Action of Haplophyllum tuberculatum Extracts

Author(s): Housseyn Chaoua, Ihcen Khacheba, Hadjer Boussoussa*, Abir Bekhaoua, Mohamed Yousfi

Journal Name: Current Enzyme Inhibition

Volume 15 , Issue 3 , 2019

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Graphical Abstract:


Background: Natural plant active compounds were found to inhibit the activity of several enzymes that may be related to several diseases.

Objective: This study aimed at testing the antidiabetic activity related to the phenol content by in vitro α-Amylase inhibitory action effect of aqueous, organic and essential oil extracts of Haplophyllum tuberculatum, collected in the town of Laghouat in the steppe region of Algeria.

Methods: Two types of aqueous extracts were prepared: Decoction and Diffusion extracts. The organic extracts were prepared with successful maceration in hexane, dichloromethane, ethyl acetate, ethanol and methanol. Also, essential oils were obtained by hydrodistillation. The analysis of the total Phenol content of our extracts was done with Folin-Ciocalteu reagent, as the flavonoid content was obtained in mixture with aluminum trichloride. The effects of the plant extracts on the catalytic efficiency of α-amylase enzyme were represented by the enzymatic inhibitory percentage of each extract in which the inhibitory activity was expressed as IC50.

Results: The total phenol content showed values ranging between 0.27 and 11.97 mg gallic acid equivalent / g dry matter. The flavonoid contents vary from 0.05 to 1.50 mg equivalent of rutin /g of dry matter. All the extracts showed good inhibitory activity against α- amylase of IC50, values ranged from 0.05 to 50.03 mg/ml.

Conclusion: This study reports for the first time the inhibitory capacity of Algerian Haplophyllum tuberculatum species against α-amylase which could provide natural biologically active agents to be used in the management of diabetes.

Keywords: Diabetes, enzyme inhibition, essential oils, Haplophyllum tuberculatum, phenolic compounds, α amylase.

Khacheba, I.; Djeridane, A.; Kameli, A.; Yousfi, M. The inhibitory effect of some algerian plants phenolics extracts on the α- glucosidase and α-amylase activities and their antioxidant activity. Curr. Enzym. Inhib., 2014, 10, 59-68.
Bekhaoua, A.; Khacheba, I.; Boussoussa, H.; Yousfi, M. α-glucosidase and α-amylase inhibitory effect and antioxidant activity of aerial part from Linaria aegyptiaca L. Curr. Enzym. Inhib., 2018, 15(1), 22-27.
Khacheba, I.; Djeridanen, A.; Yousfi, M. Twenty traditional algerian plants used in diabetes therapy as strong inhibitors of α-amylase activity. Int. J. Carbohydr. Chem., 2014, 15, 155.
Seglab, F.; Khachba, I.; Boussoussa, H.; Chaoua, H.; Djeridanen, A.; Yousfi, M. α-amylase inhibitory effect and antioxidant activity of cleome arabica depending on seasonal variations. Curr. Enzym. Inhib., 2018, 14(3), 217-225.
Boussoussa, H.; Khacheba, I.; Berramdane, T.; Maamri, A.; Bendahgane, H.; Yousfi, M. In vitro antidiabetic effect of saponins and phenolic extracts from fruits and seeds of algerian cypress tree: Cupressus sempervirens L. Curr. Enzym. Inhib., 2018, 14(2), 92-96.
Jia, W.; Gao, W.; Tang, L. Antidiabetic herbal drugs officially approved in China. Phytother. Res., 2003, 17(10), 1127-1134.
[http://dx.doi.org/10.1002/ptr.1398] [PMID: 14669243]
Al-Burtamani, S.K.S.; Fatope, M.O.; Marwah, R.G.; Onifade, A.K.; Al-Saidi, S.H. Chemical composition, antibacterial and antifungal activities of the essential oil of Haplophyllum tuberculatum from Oman. J. Ethnopharmacol., 2005, 96(1-2), 107-112.
[http://dx.doi.org/10.1016/j.jep.2004.08.039] [PMID: 15588657]
Raissi, A.; Arbabi, M.; Roustakhiz, J.; Hosseini, M. Haplophyllum tuberculatum: An overview. J HerbMed Pharmacol., 2016, 5(4), 125-130.
Eissa, T.F.; González-Burgos, E.; Carretero, M.E.; Gómez-Serranillos, M.P. Biological activity of HPLC-characterized ethanol extract from the aerial parts of Haplophyllum tuberculatum. Pharm. Biol., 2014, 52(2), 151-156.
[http://dx.doi.org/10.3109/13880209.2013.819517] [PMID: 24047505]
Sabry, O.M.; El Sayed, A.M. Potential anti-microbial, anti-inflammatory and anti-oxidant activities of Haplophyllum tuberculatum growing in libya. J. Pharmacogn. Nat. Prod., 2016, 2(1), 5-1.
Kallel, S.; Ouadday, M.Z.; Ben Ghrabi, Z. Évaluation de l’activité nématotoxique d’Haplophyllum tuberculatum sur Meloidogyne javanica. Nematol. Mediterr., 2009, 37(1), 45-52.
Khacheba, I.; Boussousssa, H.; Djeridane, A.; Bekhaoua, A.; Bensayeh, N.; Yousfi, M. α-glucosidase inhibitory effect and antioxidant activity of the extracts of eighteen plant traditionally used in algeria for diabetes. Curr. Enzym. Inhib., 2017, 13, 1-12.
Al-manhel, AJ; Niamah, AK Journal of Nutrition & Food Sciences Effect of Aqueous and Alcoholic Plant Extracts on Inhibition of Some Types of Microbes and Causing Spoilage of Food, 2015, 5-7.
Benabed, K.H.; Gourine, N.; Ouinten, M.; Bombarda, I.; Yousfi, M. Chemical composition, antioxidant and antimicrobial lamiaceae species current nutrition &. Shipin Kexue, 2017, 13, 1-13.
Singleton, V.L.; Rossi, J.A. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am. J. Enol. Vitic., 1965, 16(3), 144-158.
Djeridane, A.; Yousfi, M.; Nadjemi, B.; Maamri, S.; Djireb, F.; Stocker, P. Phenolic extracts from various Algerian plants as strong inhibitors of porcine liver carboxylesterase. J. Enzyme Inhib. Med. Chem., 2006, 21(6), 719-726.
[http://dx.doi.org/10.1080/14756360600810399] [PMID: 17252945]
Floegel, A.; Kim, D.O.; Chung, S.J.; Koo, S.I.; Chun, O.K. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J. Food Compos. Anal., 2011, 24(7), 1043-1048.
Brayer, G.D.; Luo, Y.; Withers, S.G. The structure of human pancreatic alpha-amylase at 1.8 A resolution and comparisons with related enzymes. Protein Sci., 1995, 4(9), 1730-1742.
[http://dx.doi.org/10.1002/pro.5560040908] [PMID: 8528071]
Cazzola, R.; Camerotto, C.; Cestaro, B. Anti-oxidant, anti-glycant, and inhibitory activity against α-amylase and α-glucosidase of selected spices and culinary herbs. Int. J. Food Sci. Nutr., 2011, 62(2), 175-184.
[http://dx.doi.org/10.3109/09637486.2010.529068] [PMID: 21118052]
Olubomehin, O.O.; Abo, K.A.; Ajaiyeoba, E.O. Alpha-amylase inhibitory activity of two Anthocleista species and in vivo rat model anti-diabetic activities of Anthocleista djalonensis extracts and fractions. J. Ethnopharmacol., 2013, 146(3), 811-814.
[http://dx.doi.org/10.1016/j.jep.2013.02.007] [PMID: 23422334]
Murugan, R.; Nishandhini, S.; Sudha, V.; Mallavarapu, G.R. Chemical compositions, α-amylase inhibitory and antioxidant activities of the essential oils from unripe fruit pulp and leaves of syzygium cumini. Int. J. Pharm. Pharm. Sci., 2015, 7(2), 511-514.
Biesiada, A.; Tomczak, A. Biotic and abiotic factors affecting the content of the chosen antioxidant compounds in vegetables. Veg Crop Res Bull., 2012, 76(1), 55-78.
Hamdi, A.; Viane, J.; Mahjoub, M.A.; Majouli, K.; Gad, M.H.H.; Kharbach, M.; Demeyer, K.; Marzouk, Z.; Heyden, Y.V. Polyphenolic contents, antioxidant activities and UPLC-ESI-MS analysis of Haplophyllum tuberculatum A. Juss leaves extracts. Int. J. Biol. Macromol., 2018, 106, 1071-1079.
[http://dx.doi.org/10.1016/j.ijbiomac.2017.08.107] [PMID: 28851641]
Shim, Y.; Doo, H.; Ahn, S.; Kim, Y.; Seong, J.; Park, I. Inhibitory effect of aqueous extract from the gall of Rhus chinensis on alpha-glucosidase activity and postprandial blood glucose. J. Ethnopharmacol., 2003, 85, 283-287.
Prashant, T; Bimlesh, K; Mandeep, K; Gurpreet, K; Harleen, K. Phytochemical screening and Extraction: A Review. Internationale pharmaceutica sciencia, 2011, (1)1, 106-98.
Wondra, A.G.; Lapornik, B.; Pros, M. Comparison of extracts prepared from plant by-products using different solvents and extraction time. J. Food Eng., 2005, 71, 214-222.

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Year: 2019
Page: [190 - 196]
Pages: 7
DOI: 10.2174/1573408016666191231124458
Price: $65

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