Background: Chemotherapy has shown varying success rates in the treatment of metastatic
cancer in the last 50 years. One of the problems in the use of many chemotherapeutic agents is to
increase the expression of glutathione transferase enzyme (GST; EC 184.108.40.206). Therefore, the development
of GST inhibitors is important to improve the effectiveness of antitumor drugs and to overcome
Introduction: Glutathione S-transferases (GSTs) are a major member of enzymes serving in the detoxification
of exogenous and endogenous substances. But, it has been reported that GSTs are overexpressed
in many tumour cells, and it has been found to be related to developing resistance to anticancer
drugs by these cells. The development of GST inhibitors is important to increase the efficacy
of antitumor drugs and overcome multi-drug resistance. The aim of our study was to investigate the
effect of natural compounds including curcumin, resveratrol, and quercetin on GST enzyme activity.
We also aimed to specify inhibition mechanism of the compounds on human erythrocytes GST
(hGST) with in silico study.
Methods: GST was purified from human erythrocytes using affinity chromatography (glutathione
agarose). The enzyme purity was checked with SDS-PAGE. After the inhibitory effect of the curcumin,
quercetin, resveratrol was investigated. Lastly, inhibition mechanisms of these natural compound
were identified with induced-fit docking method.
Results: GST was purified with 19.31% yield from human erythrocytes. In inhibition studies, Ki values
of curcumin, quercetin, resveratrol were determined as 0.0021 ± 0.0008, 0.0257 ± 0.0011,
663.3301 ± 0.0936 µM respectively. According to our results, all natural products showed the inhibition
effect and the order of inhibition is as follows: curcumin ˃ quercetin ˃ resveratrol.
Conclusion: According to the results of the in vitro and in silico studies, it can be said that curcumin,
quercetin, resveratrol are the inhibitors of human erythrocyte GST. In conclusion, these observations
may be of great importance for the potential use of these natural compounds as chemopreventive