Background: Although chromatography and spectrometry-based methods have been used to
analyse phenolic acids in Chinese traditional medicine Salvia miltiorrhiza Bunge (SMB), quantitative
nuclear magnetic resonance (qNMR) has never previously been used to analyse fresh SMB root extracts.
Objective: To establish a fast and simple method of quantitating danshensu, lithospermic acid, rosmarinic
acid, and salvianolic acid B content in fresh SMB root using 1H-NMR spectroscopy.
Method: Fresh SMB root was extracted using a 70% methanol aqueous solution and quantitatively
analysed for danshensu, lithospermic acid, rosmarinic acid, and salvianolic acid B using 1H-NMR
spectroscopy. Different internal standards were compared and the results were validated using highperformance
Results: The established method was accurate and precise with good recovery. The LOD and LOQ
indicated the excellent sensitivity of the method. The robustness was testified by the modification of
four different parameters, and the differences among each parameter were all less than 2%.
Conclusion: qNMR offers a fast, reliable, and accurate method of identifying and quantifying danshensu,
lithospermic acid, rosmarinic acid, and salvianolic acid B in fresh SMB root extracts.