Title:Quantitative Determination and Validation of Four Phenolic Acids in <i>Salvia Miltiorrhiza</i> Bunge using <sup>1</sup>H-NMR Spectroscopy
VOLUME: 17 ISSUE: 4
Author(s):Zhuoni Hou, Zongsuo Liang*, Yuanyuan Li, Feng Su, Jipeng Chen, Xiaodan Zhang and Dongfeng Yang
Affiliation:College of Life Sciences and Medicine, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou, College of Life Sciences and Medicine, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou, College of Life Sciences and Medicine, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou, College of Pharmaceutical Sciences, Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, Zhejiang University of Technology, Hangzhou, College of Pharmaceutical Sciences, Key Laboratory for Green Pharmaceutical Technologies and Related Equipment of Ministry of Education, Zhejiang University of Technology, Hangzhou, College of Life Sciences and Medicine, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou, College of Life Sciences and Medicine, Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang Province, Zhejiang Sci-Tech University, Hangzhou
Keywords:qNMR, Salvia Miltiorrhiza Bunge, danshensu, lithospermic acid, rosmarinic acid, salvianolic acid B.
Abstract:Background: Although chromatography and spectrometry-based methods have been used to
analyse phenolic acids in Chinese traditional medicine Salvia miltiorrhiza Bunge (SMB), quantitative
nuclear magnetic resonance (qNMR) has never previously been used to analyse fresh SMB root extracts.
Objective: To establish a fast and simple method of quantitating danshensu, lithospermic acid, rosmarinic
acid, and salvianolic acid B content in fresh SMB root using 1H-NMR spectroscopy.
Method: Fresh SMB root was extracted using a 70% methanol aqueous solution and quantitatively
analysed for danshensu, lithospermic acid, rosmarinic acid, and salvianolic acid B using 1H-NMR
spectroscopy. Different internal standards were compared and the results were validated using highperformance
liquid chromatography.
Results: The established method was accurate and precise with good recovery. The LOD and LOQ
indicated the excellent sensitivity of the method. The robustness was testified by the modification of
four different parameters, and the differences among each parameter were all less than 2%.
Conclusion: qNMR offers a fast, reliable, and accurate method of identifying and quantifying danshensu,
lithospermic acid, rosmarinic acid, and salvianolic acid B in fresh SMB root extracts.
