Introduction: Dryopteris marginalis L. is a local plant in Bangladesh which is widely
used as folk medicine.
Materials and Methods: In this study, the chemical composition of essential oil of D. marginalis L.
as well as the antioxidant activity, antibacterial activity and total phenolic content of various organic
extracts were analyzed. The gas chromatography mass spectrometry analysis was used to identify
the chemical compositions. The antibacterial activity of different extracts of Dryopteris marginalis
was examined against three gram-negative bacteria and two gram-positive bacteria. The antibacterial
activity of different extracts was investigated using the disc diffusion method and minimum inhibitory
concentration showing important antibacterial activity and was measured by using the broth
Results: The antioxidant activity was determined by using 2,2-diphenyl-1-picrylhydrazyl (DPPH)
assay and superoxide radical scavenging assay. The total phenolic portion of the extracts was ascertained
by Folin-Ciocalteu in an alkaline medium. Twenty-two volatile compounds were identified
from the essential oil. The methanol extract exhibited a significant inhibitory effect which was close
to the value of standard antibiotic (Streptomycin). The zones of inhibition of different organic extracts
against the tested bacteria were found in the range of (7-15) mm and the Minimum Inhibitory
Concentrations (MIC) were recorded (15.62-1000) μg/ml. The antioxidant activity of D. marginalis
was (82.13+1.2) % and (59.21+0.45) % highest activity with DPPH and superoxide radical scavenging
Conclusion: The methanol extract showed the highest (147.43+3.1) mg/g GAE of total phenolic content.
The extracts and essential oil of D. marginalis showing strong antibacterial activity can be considered
as a potential source of natural medicine and the ingredient of modern therapeutic agents.