Highly Sensitive Detection of CB [7] Based on Fluorescence Resonance Energy Transfer between RhB and Gold Nanoparticles

(E-pub Ahead of Print)

Author(s): Yan Zeng, Qikun Shi, Xiran Yang, Sobhan Chatterjee, Zaosheng Lv, Feng Liang*

Journal Name: Current Nanoscience

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Background: Cucurbit[n]uril (CB[n], n=5, 6, 7, 8 and 10)is a type of macrocyclic compounds formed by n glycerol units and 2n methylene. The different size of the cavity led the different chemical characteristics of CB[n]. Therefore, it was very important to distinguish and detect CB[n].

Methods: At room temperature, Rhodamine B was added with gold nanoparticles solution. The above mixture was respectively mixed with different concentrations of Cucurbituril [n] or β-cyclodextrin solutions. The fluorescence spectra and ultraviolet-visible spectra of samples were determined.

Results: It was found that 1.2nM gold nanoparticles could quench the fluorescence of 1μM Rhodamine B completely. After adding a certain amount of Cucurbituril[7], the fluorescence intensity of Rhodamine B was restored. Compared with other macrocyclic compounds, such as Cucurbituril[5], Cucurbituril[6] and β-cyclodextrin, we found that this method had unique selectivity for Cucurbituril[7]. Under the optimal conditions, the fluorescence recovery efficiency was linearly proportional to the concentration of Cucurbituril[7] in the range of 0.8-8 μg·mL-1. The detection limit was 0.21 μg·mL-1.

Conclusion: We have established an effective and practical FRET-based detection method for CB[7] with RhB as donor and the gold nanoparticles as the acceptor. The system has unique and extensively selectivity for CB[7].

Keywords: Cucurbituril[7], FRET, Gold nanoparticles, Rhodamine B, Fluorescence detection, α-cyclodextrin

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(E-pub Ahead of Print)
DOI: 10.2174/1573413716666191220152034
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