Revealing Changes in Curcumin Bioavailability using Vitamin C as an Enhancer by HPLC-MS/MS

Author(s): Xufen Dai, Jiaxue Hao, Ying Feng, Jing Wang, Qiannan Li, Cuicui Ma, Xing Wang, Zhongman Chang, Shixiang Wang, Yuxin Wang*

Journal Name: Current Pharmaceutical Analysis

Volume 17 , Issue 4 , 2021

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Graphical Abstract:


Background: Curcumin (CUR), a natural isolated compound from turmeric, helps in fighting many diseases, but the broad application of curcumin has been limited ascribed to low bioavailability.

Objective: The aim of this study is to pursue the enhancement of curcumin bioavailability through coadministration of vitamin C.

Methods: Such purpose was achieved through the analysis of curcumin pharmacokinetics by highperformance liquid chromatography coupled with electrospray ionization - tandem mass spectrometry (HPLC - ESI - MS/MS). The plasma was separated on a C18 reverse-phase column using acetonitrile and ammonium formate solution (pH 6.5; 2.0 mM) at 0.8 mL/min. MS/MS detection was carried out in negative mode using mass patterns of m/z 367.0 > 216.7 for curcumin and m/z 265.2 > 223.9 for internal standard (honokiol).

Results: Successful application of the proposed method in the pharmacokinetic study presented clear changes in key pharmacokinetic parameters, including the growth of AUC (0-t) up to 2.4 times, a 2.2- fold increase of Cmax, 2.2-fold loss of CL, and 1.5-fold diminishment of t1/2.

Conclusion: An HPLC-ESI-MS/MS method for the determination of curcumin in rat plasma and validated the improvement of bioavailability of curcumin through co-administration of vitamin C was determined. These changes were reasoned to the inhibition of lipid peroxidation induced by the use of vitamin C. Such a simple strategy is possible to become an alternative for enhancing curcumin efficiency in practice.

Keywords: Curcumin, vitamin C, antioxidants, HPLC–MS/MS, bioavailability, plasma.

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Article Details

Year: 2021
Published on: 03 March, 2021
Page: [537 - 546]
Pages: 10
DOI: 10.2174/1573412916666191220150039
Price: $65

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