Title:Pharmacokinetic Study of Zhebeirine in Mouse Blood by Ultra- Performance Liquid Chromatography/tandem Mass Spectrometry
VOLUME: 17 ISSUE: 4
Author(s):Wenhao Sun, Xiajuan Jiang, Xianqin Wang* and Xi Bao*
Affiliation:Department of Pharmacy, The Affiliated Hospital of Hangzhou Normal University, Hangzhou 310000, Department of Pharmacy, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, Analytical and Testing Center, Wenzhou Medical University, Wenzhou 325035, Department of Pharmacy, First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000
Keywords:Zhebeirine, pharmacokinetics, bioavailability, mouse, UPLC-MS/MS, blood.
Abstract:
Introduction: In this study, a precise, rapid and accurate ultra-performance liquid chromatography-
tandem mass spectrometry (UPLC-MS/MS) method for the quantification of zhebeirine in
mouse blood was developed, and pharmacokinetics of zhebeirine was studied for the first time after
intravenous and oral administration.
Methods: The mobile phase consisted of acetonitrile-0.1% formic acid, with a flow rate at 0.4 mL/m
during 4 min run time. MRM modes of m/z 414.5→81.0 for zhebeirine and m/z 430.2→412.2 for 3-
dehydroverticine (internal standard) were utilized to perform quantitative analysis. Protein in mouse
blood was directly precipitated with acetonitrile for sample preparation.
Results: The linear range was 1-3000 ng/mL with r>0.995, and LLOQ was 1 ng/mL. The intra-and
inter-day precision of zhebeirine in mouse blood was less than 13%. The accuracy ranged from 91.2%
to 112.5%, while the matrix effects were between 84.8% and 106.4%.
Conclusion: The UPLC-MS/MS was successfully applied to the pharmacokinetic study on zhebeirine
after intravenous and oral administration, and the bioavailability was determined to be 22.8%.
