Antioxidant Potency of Red Dragon Fruit Flesh and Peel Prepared by Different Methods

Author(s): Novi Febrianti, Purwanti P. Purbosari, Triana Hertiani, Sukarti Moeljopawiro, Sofia M. Haryana*

Journal Name: Current Nutrition & Food Science

Volume 16 , Issue 7 , 2020

Become EABM
Become Reviewer
Call for Editor

Graphical Abstract:


Background: Red dragon fruit (Hylocereus polyrhizus, (F.A.C. Weber) Britton & Rose) is widely consumed all over the world nowadays. The peel and flesh of red dragon fruit contain many bioactive compounds with high antioxidant activity. The preparation process is critical to maximizing the yield of the antioxidant content.

Objective: The objectives of this research were to evaluate total phenolic content (TPC), total flavonoid content (TFC), as well as the antioxidant activity of peel and flesh of red dragon fruit prepared by various methods.

Methods: The fresh and dried samples of peel and flesh of red dragon fruit were prepared via maceration and non-maceration process. Ethanol (96%) was used as the solvent in maceration. In the nonmaceration process, the samples were ground using a blender and pressed using a juicer. TPC was analyzed by Folin-Ciocalteau methods, while TFC was determined by spectrophotometry UV-Vis with AlCl3. Antioxidant activity was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene bleaching (BCB) tests.

Results: TPC from all of the measured samples varied from 22.43 ± 0.27 to 80.54 ± 0.43 mg GAE/g dry extract. The highest TPC concentration was found in the blended peel via maceration and the lowest concentration was found in the blended flesh without maceration. The dried peel via maceration treatment had the highest TFC (51.96 ± 0.084 mg of QE/g dry extract). Regarding to the antioxidant activity, the blended flesh ethanolic extract and blended peel ethanolic extract had the highest DPPH radical scavenging, IC50=966.83 ± 11.62 and 973.81 ± 3.571ppm, respectively. While the blended peel ethanolic extract had the highest BCB antioxidant activity (IC50= 45.48 ± 6,79 mg/mL).

Conclusion: Preparation methods affect the antioxidant activity of red dragon fruit peel and flesh. The highest TPC and antioxidant activity (BCB test) can be found in the ethanolic extract of the blended peel. The highest TFC can be found in the ethanolic extract of dried-peel. Both the ethanolic extracts, blended peel and blended flesh, had the same DPPH radical scavenging activity.

Keywords: Antioxidant activity, flavonoid, natural health, phenol, preparation method, red dragon fruit.

Choo JC, Koh RY, Pick A, Ling K, Spatula DD. Medicinal properties of Pitaya: a review Pitaya’nın tedavi edici özelliği: derleme. Rev Artic Spat DD 2016; 6: 69-76.
Ramli NS, Brown L, Ismail P, Rahmat A. Effects of red pitaya juice supplementation on cardiovascular and hepatic changes in high-carbohydrate, high-fat diet-induced metabolic syndrome rats. BMC Complement Altern Med 2014; 14: 189.
[] [PMID: 24919841]
Omidizadeh A, Yusof RM, Roohinejad S, et al. Anti-diabetic activity of red pitaya (Hylocereus polyrhizus) fruit. RSC Advances 2014; 4: 62978-86.
Macias-Ceja DC, Cosín-Roger J, Ortiz-Masiá D, et al. The flesh ethanolic extract of Hylocereus polyrhizus exerts anti-inflammatory effects and prevents murine colitis. Clin Nutr 2016; 35(6): 1333-9.
[] [PMID: 26948401]
Hernawati, Setiawan NA, Shintawati R, Priyandoko D. The role dragon fruit peel (Hylocereus polyrhizus) to improvement blood lipid levels of hyperlipidemia male mice. J Phys 2018; 12167.
Suh DH, Lee S, Heo Y, et al. Metabolite profiling of red and white pitayas (Hylocereus polyrhizus and Hylocereus undatus) for comparing betalain biosynthesis and antioxidant activity. J Agric Food Chem 2014; 62(34): 8764-71.
[] [PMID: 25101804]
Sim Choo W, Khing Yong W. Antioxidant properties of two species of Hylocereus fruits. Adv Appl Sci Res 2011; 2: 418-25.
Nurliyana R, Syed Zahir I, Mustapha Suleiman K, Aisyah MR, Kamarul Rahim K. Antioxidant study of pulps and peels of dragon fruits: A comparative study. Int Food Res J 2010; 17: 367-75.
Azwanida N. A review on the extraction methods use in medicinal plants, principle, strength and limitation. Med Aromat Plants 2015; 4: 3-8.
Maria Burin V, Ferreira-Lima NE, Panceri CP, Bordignon-Luiz MT. Bioactive compounds and antioxidant activity of Vitis vinifera and Vitis labrusca grapes: Evaluation of different extraction methods. 2014.
Ramli NS, Ismail P, Rahmat A. Influence of conventional and ultrasonic-assisted extraction on phenolic contents, betacyanin contents, and antioxidant capacity of red dragon fruit (Hylocereus polyrhizus). Sci World J 2014.: Article ID 2014964731.
[] [PMID: 25379555]
Stankovic M, Niciforovic N, Topuzofic M, Solujic S. Total Phenolic Content, Flavonoid Concentration, and Antioxidant Activity of the Whole Plant and Plant Parts Extracts from Teucrium Montanum L. Var Montanum, F. Supinum (L.) Reichenb. Biotechnol Biotechnol Equip 2011; 25: 2222-7.
Azizah DN, Kumolowati E, Faramayuda F. Penetapan kadar flavonoid metode AlCl3 pada ekstrak metanol kulit buah kakao (Theobroma caco L.). Kartika J Ilm Farm 2014; 2: 45-9.
Kulisic T, Radonic A, Katalinic V, Milos M. Use of different methods for testing antioxidative activity of oregano essential oil. Food Chem 2004; 85: 633-40.
Dobiáš P, Pavlíková P, Adam M, Eisner A, Beňová B, Ventura K. Comparison of pressurised fluid and ultrasonic extraction methods for analysis of plant antioxidants and their antioxidant capacity. Open Chem 2010; 8: 87-95.
Kim H, Choi H-K, Moon JY, Kim YS, Mosaddik A, Cho SK. Comparative Antioxidant and Antiproliferative Activities of Red and White Pitayas and Their Correlation with Flavonoid and Polyphenol Content n.d.
Wu L, Hsu H-W, Chen Y-C, Chiu C-C, Lin Y-I, Ho JA. Antioxidant and antiproliferative activities of red pitaya. Food Chem 2006; 95: 319-27.
Paniwnyk L, Alarcon-Rojo A, Rodriguez-Figueroa JC, Toma M. The Use of Ultrasound as an Enhancement Aid to Food Extraction 2017.
Ueno H, Yamakura S, Arastoo RS, Oshima T, Kokubo K. Systematic evaluation and mechanistic investigation of antioxidant activity of fullerenols using β -Carotene bleaching assay. J Nanomater 2014 2014.
Matsusaka Y, Kawabata J. Evaluation of antioxidant capacity of non-edible parts of some selected tropical fruits. Food Sci Technol Res 2010; 16: 467-72.
Antioxidant Potency of Red Dragon Fruit Flesh and Peel Prepared by Different Methods - Repository Universitas Ahmad Dahlan. n.d.

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2020
Page: [1106 - 1111]
Pages: 6
DOI: 10.2174/1573401316666191216124950
Price: $65

Article Metrics

PDF: 25