Exosomal miR-1298 and lncRNA-RP11-583F2.2 Expression in Hepatocellular Carcinoma

Author(s): Marwa Matboli*, Mariam E. Labib, Hanaa El-Tayeb Nasser, Ahmed H.F. El-Tawdi, Eman K. Habib, Randa Ali-Labib

Journal Name: Current Genomics

Volume 21 , Issue 1 , 2020


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Abstract:

Aim: The aim of this study was to explore the expression of exosomal non-coding RNAs (ncRNAs) in the sera of patients with HCC versus control.

Methods: Firstly, Bioinformatics analysis was conducted to retrieve ncRNAs specific to HCC (hsamiRNA- 1298 and lncRNA-RP11-583F2.2). Afterwards, extraction and characterization of exosomes were performed. We measured the expression of the chosen exosomal RNAs by reverse transcriptase quantitative real-time PCR in sera of 60 patients with HCC, 42 patients with chronic hepatitis C (CHC) infection and 18 healthy normal volunteers.

Results: The exosomal ncRNAs [hsa-miRNA-1298, lncRNA-RP11-583F2.2] had better sensitivity and specificity than alpha-fetoprotein (AFP) in HCC diagnosis.

Conclusion: The exosomal hsa-miRNA-1298, lncRNA-RP11-583F2.2 can be potential biomarkers for HCC diagnosis.

Keywords: Hepatocellular carcinoma, exosome, miRNA, lncRNA, diagnosis, chronic hepatitis C.

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Article Details

VOLUME: 21
ISSUE: 1
Year: 2020
Published on: 09 December, 2019
Page: [46 - 55]
Pages: 10
DOI: 10.2174/1389202920666191210111849
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