Aim: The aim of this study was to explore the expression of exosomal non-coding RNAs
(ncRNAs) in the sera of patients with HCC versus control.
Methods: Firstly, Bioinformatics analysis was conducted to retrieve ncRNAs specific to HCC (hsamiRNA-
1298 and lncRNA-RP11-583F2.2). Afterwards, extraction and characterization of exosomes
were performed. We measured the expression of the chosen exosomal RNAs by reverse transcriptase
quantitative real-time PCR in sera of 60 patients with HCC, 42 patients with chronic hepatitis C
(CHC) infection and 18 healthy normal volunteers.
Results: The exosomal ncRNAs [hsa-miRNA-1298, lncRNA-RP11-583F2.2] had better sensitivity
and specificity than alpha-fetoprotein (AFP) in HCC diagnosis.
Conclusion: The exosomal hsa-miRNA-1298, lncRNA-RP11-583F2.2 can be potential biomarkers for