Synbiotic Functional Malt Beverage Supplemented with Inulin and Probiotic Strains

Author(s): Nayereh Zakipour, Leila R. Nasiraie, Amir M. Mortazavian, Nasim Khorshidian, Sara Sohrabvandi*

Journal Name: Current Nutrition & Food Science

Volume 16 , Issue 7 , 2020


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Graphical Abstract:


Abstract:

Background: Cereal-based beverages can provide essential nutrients and phytochemicals as well as being suitable carriers for probiotics. Prebiotic ingredients present in grains or incorporated into beverages can improve the growth of probiotics leading to the emergence of functional synbiotic beverages.

Objective: This study aimed to produce a malt-based synbiotic beverage containing inulin as a prebiotic and three strains of probiotics and evaluate its physiochemical and antioxidant properties during storage.

Methods: A synbiotic malt beverage containing 2.25% inulin and 2.25% sucrose with three probiotic strains including Lactobacillus acidophilus, Lactobacillus casei and Lactobacillus plantarum was produced and its chemical properties, antioxidant activity, total phenolic compounds and viability of probiotics were determined during 28 days of storage.

Results: The results showed that although the viability of probiotic bacteria in synbiotic malt beverage was decreased during refrigerated storage, it remained above 107 cfu/mL. The lowest and the highest viability was observed in the case of L. acidophilus and L. plantarum, respectively after 28 days of storage. pH, brix and total sugar decreased due to probiotic growth and activity. Ethanol concentration increased, but was lower than 0.1% in all samples and thus the final product can be considered as non-alcoholic. Moreover, the content of phenolic compounds was increased in samples with probiotics due to the hydrolysis of glycosylic phenolic compounds and the generation of free phenolic compounds. The samples containing L. casei presented the highest total phenolic content followed by L. acidophilus and L. plantarum, respectively. Malt beverages containing L. casei showed the highest antioxidant activity followed by samples with L. acidophilus and L. plantarum.

Conclusion: It can be concluded that proper probiotic strains besides inulin as a prebiotic can be used for the development of a functional synbiotic malt beverage with high phenolic content and antioxidant activity.

Keywords: Antioxidant activity, inulin, malt beverage, probiotics, synbiotic, viability.

[1]
Paseephol T, Sherkat F. Probiotic stability of yoghurts containing Jerusalem artichoke inulins during refrigerated storage. J Funct Foods 2009; 1: 311-8.
[http://dx.doi.org/10.1016/j.jff.2009.07.001]
[2]
Yew GY, Lee SY, Show PL, et al. Recent advances in algae biodiesel production: From upstream cultivation to downstream processing. Bioresource Technol Rep 2019., 7100227.
[http://dx.doi.org/10.1016/j.biteb.2019.100227]
[3]
Khoo KS, Lee SY, Ooi CW, et al. Recent advances in biorefinery of astaxanthin from Haematococcus pluvialis. Bio resour Technol 2019; 288, : 121606..
[http://dx.doi.org/10.1016/j.biortech.2019.121606] [PMID: 31178260]
[4]
Shanahan F. Probiotics in inflammatory bowel disease--therapeutic rationale and role. Adv Drug Deliv Rev 2004; 55(6): 809-18.
[http://dx.doi.org/10.1016/j.addr.2003.11.003] [PMID: 15063591]
[5]
Hill C, Guarner F, Reid G, et al. Expert consensus document. The International Scientific Association for Probiotics and Prebiotics consensus statement on the scope and appropriate use of the term probiotic. Nat Rev Gastroenterol Hepatol 2014; 11(8): 506-14.
[http://dx.doi.org/10.1038/nrgastro.2014.66] [PMID: 24912386]
[6]
Mortazavian AM, Ehsani MR, Mousavi SM, Reinheimer JA, Sohrabvandi S, Rezaei K. Preliminary investigation of the combined effect of heat treatment and incubation temperature on the viability of the probiotic micro-organisms in freshly made yogurt. Int J Dairy Technol 2006; 59: 8-11.
[http://dx.doi.org/10.1111/j.1471-0307.2006.00216.x]
[7]
Al-Sheraji SH, Ismail A, Manap MY, Mustafa S, Yusof RM, Hassan FA. Prebiotics as functional foods: A review. J Funct Foods 2013; 5(4): 1542-53.
[http://dx.doi.org/10.1016/j.jff.2013.08.009]
[8]
Guimarães JT, Silva EK, Costa AL, et al. Manufacturing a prebiotic whey beverage exploring the influence of degree of inulin polymerization. Food Hydrocoll 2018; 77: 787-95.
[http://dx.doi.org/10.1016/j.foodhyd.2017.11.021]
[9]
Corbo MR, Bevilacqua A, Petruzzi L, Casanova FP, Sinigaglia M. Functional beverages: The emerging side of functional foods. Compr Rev Food Sci 2014; 13: 1192-206.
[http://dx.doi.org/10.1111/1541-4337.12109]
[10]
Enujiugha VN, Badejo AA. Probiotic potentials of cereal-based beverages. Crit Rev Food Sci Nutr 2017; 57(4): 790-804.
[http://dx.doi.org/10.1080/10408398.2014.930018] [PMID: 26558644]
[11]
Dimitrovski D, Velickova E, Dimitrovska M, Langerholc T, Winkelhausen E. Synbiotic functional drink from Jerusalem artichoke juice fermented by probiotic Lactobacillus plantarum PCS26. J Food Sci Technol 2016; 53(1): 766-74.
[http://dx.doi.org/10.1007/s13197-015-2064-0] [PMID: 26787997]
[12]
Sip A, Grajek W. Probiotics and prebiotics. In: Smith J, Charter E, Eds. Functional food product development. 1st ed. New York: John Wiley & Sons 2009; pp. 147-77.
[13]
Granato D, Branco GF, Nazzaro F, Cruz AG, Faria JA. Functional foods and nondairy probiotic food development: Trends, concepts, and products. Compr Rev Food Sci 2010; 9: 292-302.
[http://dx.doi.org/10.1111/j.1541-4337.2010.00110.x]
[14]
Prado FC, Parada JL, Pandey A, Soccol CR. Trends in non-dairy probiotic beverages. Food Res Int 2008; 41: 111-23.
[http://dx.doi.org/10.1016/j.foodres.2007.10.010]
[15]
Sohrabvandi S, Mortazavian AM, Rezaei K. Health-related aspects of beer: a review. Int J Food Prop 2012; 15: 350-73.
[http://dx.doi.org/10.1080/10942912.2010.487627]
[16]
Ahmadi E, Mortazavian AM, Mohammadi R. The effect of heat inactivating process on biochemical, microbiological and sensory characteristics of Iranian drink based on fermented milk (Doogh). J Food Process Technol 2013; 4: 1-4.
[17]
Mortazavian AM, Khosrokhavar R, Rastgar H. Effects of dry matter standardization order on biochemical and microbiological characteristics of Doogh (Iranian fermented milk drink). Ital J Food Sci 2010; 1: 98-104.
[18]
Ruberto G, Renda A, Daquino C, et al. Polyphenol constituents and antioxidant activity of grape pomace extracts from five Sicilian red grape cultivars. Food Chem 2007; 100: 203-10.
[http://dx.doi.org/10.1016/j.foodchem.2005.09.041]
[19]
Yoon KY, Woodams EE, Hang YD. Fermentation of beet juice by beneficial lactic acid bacteria. Lebensm Wiss Technol 2005; 37: 73-5.
[http://dx.doi.org/10.1016/j.lwt.2004.04.008]
[20]
Gupta S, Cox S, Abu-Ghannam N. Process optimization for the development of a functional beverage based on lactic acid fermentation of oats. Biochem Eng J 2010; 52: 199-204.
[http://dx.doi.org/10.1016/j.bej.2010.08.008]
[21]
Rozada R, Vázquez JA, Charalampopoulos D, Thomas K, Pandiella SS. Effect of storage temperature and media composition on the survivability of Bifidobacterium breve NCIMB 702257 in a malt hydrolisate. Int J Food Microbiol 2009; 133(1-2): 14-21.
[http://dx.doi.org/10.1016/j.ijfoodmicro.2009.04.003] [PMID: 19539389]
[22]
Kailasapathy K. Survival of free and encapsulated probiotic bacteria and their effect on the sensory properties of yoghurt. Lebensm Wiss Technol 2006; 39: 1221-7.
[http://dx.doi.org/10.1016/j.lwt.2005.07.013]
[23]
Shah NP, Lankaputhra WE, Britz ML, Kyle WS. Survival of Lactobacillus acidophilus and Bifidobacterium bifidum in commercial yoghurt during refrigerated storage. Int Dairy J 1995; 5: 515-21.
[http://dx.doi.org/10.1016/0958-6946(95)00028-2]
[24]
Pereira ALF, Almeida FDL, de Jesus ALT, da Costa JMC, Rodrigues S. Storage stability and acceptance of probiotic beverage from cashew apple juice. Food Bioprocess Technol 2013; 6: 3155-65.
[http://dx.doi.org/10.1007/s11947-012-1032-1]
[25]
Mashayekh S, Hashemiravan M, Mokhtari FD. Study on chemical and sensory changes of probiotic fermented beverage based on mixture of pineapple, apple and mango juices. J Curr Res Sci 2016; 4: 1-5.
[26]
Ghasemi S, Zomorodi S. The effect of capsulation on survival of Lactobacillus acidophilus and qualitative properties of apple juice during storage at ambient temperature. J Food Sci Nutr 2014; 2: 81-90.
[27]
Kedia G, Wang R, Patel H, Pandiella SS. Use of mixed cultures for the fermentation of cereal-based substrates with potential probiotic properties. Process Biochem 2007; 42: 65-70.
[http://dx.doi.org/10.1016/j.procbio.2006.07.011]
[28]
Pereira AP, Mendes‐Ferreira A, Estevinho LM, Mendes‐Faia A. Improvement of mead fermentation by honey-must supplementation. J I Brewing 2015; 121(3): 405-10.
[29]
Curiel JA, Rodriguez H, Landete JM, De las Rivas B, Munoz R. Ability of Lactobacillus brevis to degrade food phenolic acids. Food Chem 2010; 120: 225-9.
[http://dx.doi.org/10.1016/j.foodchem.2009.10.012]
[30]
Vuong T, Martin L, Matar C. Antioxidant activity of fermented berry juices and their effects on nitric oxide and tumor necrosis ractor‐alpha production in macrophages 264.7 gamma no (–) cell line. J Food Biochem 2006; 30: 249-68.
[http://dx.doi.org/10.1111/j.1745-4514.2006.00054.x]
[31]
Frumento D, do Espirito Santo AP, Aliakbarian B, et al. Development of milk fermented with Lactobacillus acidophilus Fortified with Vitis vinifera marc flour. Food Technol Biotechnol 2013; 51: 370-5.
[32]
Kumar BV, Sreedharamurthy M, Reddy O. Probiotication of mango and sapota juices using Lactobacillus plantarum NCDC LP 20. Nutrafoods 2015; 14: 97-106.
[http://dx.doi.org/10.1007/s13749-015-0002-4]
[33]
Khanniri E, Sohrabvandi S, Mortazavian AM, Khorshidian N, Malganjim S. Effect of fermentation, cold storage and carbonation on the antioxidant activity of probiotic grape beverage. Curr Nutr Food Sci 2018; 14: 335-40.
[http://dx.doi.org/10.2174/1573401313666170614100418]
[34]
Pyrzynska P, Pekal A. Application of free radical diphenylpicrylhydrazyl (DPPH) to estimate the antioxidant capacity of food samples. Anal Methods 2013; 5: 4288-95.
[http://dx.doi.org/10.1039/c3ay40367j]
[35]
Chen Z, Bertin R, Froldi G. EC50 estimation of antioxidant activity in DPPH. assay using several statistical programs. Food Chem 2013; 138(1): 414-20.
[http://dx.doi.org/10.1016/j.foodchem.2012.11.001] [PMID: 23265506]
[36]
Katina K, Liukkonen KH, Kaukovirta-Norja A, et al. Fermentation induced changes in the nutritional value of native or germinated rye. J Cereal Sci 2007; 46: 348-55.
[http://dx.doi.org/10.1016/j.jcs.2007.07.006]
[37]
Lu Y, Yeap Foo L. Antioxidant and radical scavenging activities of polyphenols from apple pomace. Food Chem 2000; 68: 81-5.
[http://dx.doi.org/10.1016/S0308-8146(99)00167-3]
[38]
Fazeli MR, Bahmani S, Jamalifar H, Samadi N. Effect of probiotication on antioxidant and antibacterial activities of pomegranate juices from sour and sweet cultivars. Nat Prod Res 2011; 25(3): 288-97.
[http://dx.doi.org/10.1080/14786419.2010.495068] [PMID: 21294041]


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Article Details

VOLUME: 16
ISSUE: 7
Year: 2020
Page: [1098 - 1105]
Pages: 8
DOI: 10.2174/1573401315666191209145851
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