Dual Inhibition of DPP-4 and Cholinesterase Enzymes by the Phytoconstituents of the Ethanolic Extract of Prosopis cineraria Pods: Therapeutic Implications for the Treatment of Diabetes-associated Neurological Impairments

Author(s): Heera Ram*, Noopur Jaipal, Pramod Kumar, Purbajyoti Deka, Shivani Kumar, Priya Kashyap, Suresh Kumar, Bhim P. Singh, Abdulaziz A. Alqarawi, Abeer Hashem, Baby Tabassum, Elsayed Fathi Abd_Allah

Journal Name: Current Alzheimer Research

Volume 16 , Issue 13 , 2019

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Abstract:

Background: Insulin resistance causes decreased uptake of glucose which promotes the susceptibility of type 2 associated neurological impairments.

Methods: The study was aimed to evaluate the inhibition potential of the ethanolic extract of Prosopis cineraria (EPC) pods against DPP-4 and cholinesterase enzymes by in-vitro, in-vivo and in-silico assessments. The present study consists of in vivo studies on a diabetes-induced rat model by HOMA (Homeostasis model assessment) and related parameters, in vitro studies through the DPP-4 enzyme assay and cholinesterase assays using Ellman’s reaction. The in-silico studies were conducted by the molecular docking of Cinerin C with targeted enzymes. The phytochemical characterization of the extract was demonstrated through LCMS studies. The antioxidant studies on the extract were performed by FRAP and TEAC assays.

Results: The extract showed 64.8% maximum inhibition of DPP-4, 34.91% inhibition of AChE and 74.35% inhibition of BuChE. The antioxidant capacity of the extract was observed to be 847.81±16.25μM Fe2+ equivalent in the FRAP assay and 0.40 ± 0.08 mmol/l of Trolox equivalent in the TEAC assay. The in vivo study showed competent glycaemic control against significant HOMA IR (1.5), HOMA % β (26.5) and HOMA % S (68.8) as well as pancreatic cell mass proliferation. The insilico analysis also revealed positive interactions of Cinerin C with targeted enzymes (DPP4 and cholinesterase).

Conclusion: It can be concluded that the phytoconstituents of Prosopis cineraria pod extract can be significantly considered in neuropharmacology to resolve insulin resistance-induced neurological complications as it showed inhibition against DPP-4, AChE and BuChE target enzymes.

Keywords: Prosopis cineraria, diabetes mellitus, Alzheimer’s disease, DPP-4, acetylcholinesterase (AChE), butyrylcholinesterase (BuChE).

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Article Details

VOLUME: 16
ISSUE: 13
Year: 2019
Page: [1230 - 1244]
Pages: 15
DOI: 10.2174/1567205016666191203161509
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