Background: Cockle shell-derived calcium carbonate nanoparticles (CSCaCO3NP) are
natural biogenic inorganic material that is used in drug delivery mainly as a bone-remodeling agent
as well as a delivery agent for various therapeutics against bone diseases. Curcumin possesses a
wide safety margin and yet puzzled with the problem of poor bioavailability due to insolubility.
Propounding in vitro and in vivo studies on toxicity assessments of newly synthesized nanoparticles
are ongoing to overcome some crucial challenges regarding their safety administration. Nanotoxicology
has paved ways for concise test protocols to monitor sequential events with regards to
possible toxicity of newly synthesized nanomaterials. The development of nanoparticle with no or
less toxic effect has gained tremendous attention.
Objective: This study aimed at evaluating the in vitro cytotoxic effect of curcumin-loaded cockle
shell-derived calcium carbonate nanoparticles (Cur-CSCaCO3NP) and assessing its biocompatibility
on normal cells using standard techniques of WST’s assay.
Methods: Standard techniques of WST’s assay was used for the evaluation of the biocompatibility
Results: The result showed that CSCaCO3NP and Cur-CSCaCO3NP possess minimal toxicity and
high biocompatibility on normal cells even at a higher dose of 500 μg/ml and 40 μg/ml, respectively.
Conclusion: CSCaCO3NP can be termed an excellent non-toxic nanocarrier for curcumin delivery.
Hence, curcumin loaded cockle shell derived calcium carbonate nanoparticles (Cur-CSCaCO3NP)
could further be assessed for various in vivo and in vitro therapeutic applications against various
bone- related ailments.