Nutritional Composition and Qualitative Phytochemical Analysis of Chia Seeds (Salvia hispanica L.) Grown in East Africa

Author(s): Fabian D. Mihafu*, Beatrice N. Kiage, Judith K. Okoth, Andrew K. Nyerere

Journal Name: Current Nutrition & Food Science

Volume 16 , Issue 6 , 2020

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Graphical Abstract:


Background: Chia seed (Salvia hispanica L.) is becoming one of the most popular plantbased foods that contain the greatest amount of nutrients particularly omega-3 fatty acid, α-linolenic acid. It is therefore considered a functional food with pronounced health benefits.

Objective: The purpose of this study was to determine the proximate composition, minerals, fatty acid profiles and phytochemical contents of chia seeds grown in East Africa (Kenya and Uganda).

Methods: Official methods of analysis, 2005 were adopted, minerals determined by Atomic absorption spectrophotometer, phytochemicals were determined by standard methods and fatty acid profiles were analyzed by Gas chromatography.

Results: Proximate composition indicated high contents of protein, fat and fiber. The fatty acid profiles revealed great amounts of α-linolenic acid (45.29-56.99%) followed by linoleic acid (15.9- 20.28%) and oleic acid (6.88-11.58%). However, the difference in the content of α-linolenic acid between samples was not significant (p = 0.7391). Mineral determination (mg/100g) showed high contents of potassium (492.96-862.98), phosphorous (486.45-569.45), calcium (297.47-429.09) and magnesium (192.22-202.97) while considerable amount was observed for iron, zinc, manganese, and copper. There was a significant difference (p = 0.0001) in mineral content between black chia Molo and white chia Bukembo with the exception of phosphorus.

Conclusion: Both black and white chia seeds grown in East Africa, observed to have high amounts of α-linolenic acid, proteins, fats, fiber, and minerals. These findings support the evidence that chia is rich in nutrients that are beneficial to human health. Therefore, we suggest its incorporation in diets as a healthy food ingredient.

Keywords: Fatty acids, functional food, minerals, phytochemicals, proximate composition, vitamins.

Alissa EM, Ferns GA. Functional foods and nutraceuticals in the primary prevention of cardiovascular diseases. J Nutr Metab 2012; 2012: 1-6.
[] [PMID: 22570771]
Ixtaina VY, Nolasco SM, Tomás MC. Physical properties of chia (Salvia hispanica L.) seeds. Ind Crops Prod 2008; 28: 286-93.
Mohd Ali N, Yeap SK, Ho WY, Beh BK, Tan SW, Tan SG. The promising future of chia, Salvia hispanica L. J Biomed Biotechnol 2012; 2012171956
[] [PMID: 23251075]
Bochicchio R, Philips TD, Lovelli S, et al. Innovative crop productions for healthy food: the case of chia (Salvia hispanica L). In: Vastola A, EdThe sustainability of agro-food and natural resource systems in the Mediterranean basin Switzerland. Springer: Cham 2015; pp. 29-45.
Yeboah S, Owusu Danquah E, Lamptey JNL, et al. Influence of planting methods and density on performance of chia (Salvia hispanica) and its suitability as an oilseed plant. Agric Sci 2014; 2(4): 14-26.
Antruejo A, Azcona JO, Garcia PT, et al. Omega-3 enriched egg production: the effect of α -linolenic ω -3 fatty acid sources on laying hen performance and yolk lipid content and fatty acid composition. Br Poult Sci 2011; 52(6): 750-60.
[] [PMID: 22221241]
Ayerza R, Coates W. Ground chia seed and chia oil effects on plasma lipids and fatty acids in the rat. Nutr Res 2005; 25: 995-1003.
Segura-Campos MR, Ciau-Solís N, Rosado-Rubio G, Chel-Guerrero L, Betancur-Ancona D. Chemical and functional properties of chia seed (Salvia hispanica L.) gum. Int J Food Sci 2014; 20141-5
[] [PMID: 26904622]
Creus A, Ferreira MR, Oliva ME, Lombardo YB. Mechanisms involved in the improvement of lipotoxicity and impaired lipid metabolism by dietary α-linolenic acid rich Salvia hispanica L (Salba) seed in the heart of dyslipemic insulin-resistant rats. J Clin Med 2016; 5(2): 18.
[] [PMID: 26828527]
Amato M, Caruso MC, Guzzo F, et al. Nutritional quality of seeds and leaf metabolites of chia (Salvia hispanica L.) from Southern Italy. Eur Food Res Technol 2015; 241: 615-25.
Giaretta D, Lima VA, Carpes ST. Improvement of fatty acid profile in breads supplemented with Kinako flour and chia seed. Innov Food Sci Emerg Technol 2018; 49: 211-4.
Julio LM, Capitani MI, Guiotto EN, Ixtaina VY, Tomás MC, Nolasco SM. Characterization of chia (Salvia hispanica L) seed and byproducts from Argentina. In: Betancur D, Segura-Campos M, Eds Saliva hispanica L: properties, application and health. UK:: Nova Publishers 2016;; pp. 87- 106..
Foss J. Building a global supply chain from farm to consumers. The Chia Company Australian Grains Industry Conference 2014 .
Suri S, Passi JS, Goyat J. chia seed (salvia hispanica l) – a new age functional food 2016 Proceedings of the 4th International Conference on Recent Innovation in Science Engineering and Management 2016. March 20; New Delhi, India: India International Centre
Ayerza(h) R, Coates W. Influence of environment on growing period and yield, protein, oil and α-linolenic content of three chia (Salvia hispanica L.) selections. Ind Crops Prod 2009; 30: 321-4.
Ayerza R. Effects of seed color and growing locations on fatty acid content and composition of two chia (Salvia hispanica L.) genotypes. J Am Oil Chem Soc 2010; 87: 1161-5.
Menga V, Amato M, Phillips TD, Angelino D, Morreale F, Fares C. Gluten-free pasta incorporating chia (Salvia hispanica L.) as thickening agent: An approach to naturally improve the nutritional profile and the in vitro carbohydrate digestibility. Food Chem 2017; 221: 1954-61.
[] [PMID: 27979185]
AOAC. Official Methods of Analysis of AOAC.In: Official Methods of Analysis of AOAC International, . 2005.
Hart FL, Fisher HJ. Introduction-general methods for proximate and mineral analysis.In Modern food analysis2012. New York: Springer-Verlag Inc. 2012; pp. 1-2.
García R, Báez P. Atomic Absorption Spectrometry (AAS).n: Farrukh MA, EdAtomic Absorption Spectrometry. UK: IntechOpen 2012; pp. 1-14.
Okalebo JR, Gathua KW, Woomer PL. Laboratory methods of soil and plant analysis: a working manual. Nairobi: Tropical Soil Biology and Fertility Programme 1993.
Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol 1959; 37(8): 911-7.
[] [PMID: 13671378]
Banu K, Cathrine L. General techniques involved in phytochemical analysis. Int J Adv Res Chem Sci 2015; 2(4): 25-32.
Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Int Pharm Sci 2011; 1(1): 98-106.
Thakur S, Sidhu MC. Phytochemical screening of some traditional medicinal plants. Res J Pharm Biol Chem Sci 2014; 5: 1088-97.
Sargi SC, Silva BC, Santos HMC, et al. Antioxidant capacity and chemical composition in seeds rich in omega-3: chia, flax, and perilla. Food Sci Technol 2013; 33(3): 541-8.
Owaga E, Kibui AN, Mburu M. Proximate composition and nutritional characterization of chia enriched yoghurt. Afr J Food Agric Nutr Dev 2018; 18(1): 13239-53.
Marineli R da S, Moraes EA, Lenquiste SA, Godoy AT, Eberlin MN, Maróstica MR. Jr. Chemical characterization and antioxidant potential of Chilean chia seeds and oil (Salvia hispanica L.). Lebensm Wiss Technol 2014; 59: 1304-10.
Coelho MS, de las Mercedes Salas-Mellado M. Chemical characterization of chia (Salvia hispanica L.) for use in food products. J Food Nutr Res 2014; 2(5): 263-9.
Ullah R, Nadeem M, Khalique A, et al. Nutritional and therapeutic perspectives of Chia (Salvia hispanica L.): a review. J Food Sci Technol 2016; 53(4): 1750-8.
[] [PMID: 27413203]
Ding Y, Lin HW, Lin YL, et al. Nutritional composition in the chia seed and its processing properties on restructured ham-like products. Yao Wu Shi Pin Fen Xi 2018; 26(1): 124-34.
[] [PMID: 29389547]
Saphier O, Silberstein T, Kamer H, Ben-Abu Y, Tavor D. Chia seeds are richer in polyphenols compared to flax seeds. Integr Food Nutr Metab (Lond) 2017; 4(3): 1-4.
Ayerza(h) R. Seed composition of two chia (Salvia hispanica L.) genotypes which differ in seed color. Emir J Food Agric 2013; 25(7): 495-500.
Segura-Campos MR, Ciau-Solís N, Rosado-Rubio G, Chel-Guerrero L, Betancur-Ancona D. Physicochemical characterization of chia (Salvia hispanica) seed oil from Yucatán, México. Agric Sci 2014; 5(3): 220-6.
de Souza Ferreira C. dd Sousa Fomes LdeF, da Silva GE, Rosa G. Effect of chia seed (Salvia hispanica L.) consumption on cardiovascular risk factors in humans: A systematic review. Nutr Hosp 2015; 32(5): 1909-18.
[PMID: 26545644]
Ramzi AAG, Sharada C. Omega fatty acids in health and disease: a review. J Pharm Res 2014; 8(8): 1027-44.
Mozaffarian D, Appel LJ, Van Horn L. Components of a cardioprotective diet: new insights. Circulation 2011; 123(24): 2870-91.
[] [PMID: 21690503]
Liu RH. Health-promoting components of fruits and vegetables in the diet. Adv Nutr 2013; 4(3): 384S-92S.
[] [PMID: 23674808]
Liu RH. Potential synergy of phytochemicals in cancer prevention: mechanism of action. J Nutr 2004; 134(12)(Suppl.): 3479S-85S.
[] [PMID: 15570057]
Vijay R, Sharmila KP, Suchetha Kumari N. In vitro evaluation of phytochemical and antioxidant activity of aqueous seed extract of Salvia hispanica L. (chia). World J Pharm Pharm Sci 2014; 3(6): 776-89.
Liu RH. Dietary bioactive compounds and their health implications. J Food Sci 2013; 78(S1)(Suppl. 1): A18-25.
[] [PMID: 23789932]
Felisberto MHF, Wahanik AL, Gomes-Ruffi CR, Clerici MTPS, Chang YK, Steel CJ. Use of chia (Salvia hispanica L.) mucilage gel to reduce fat in pound cakes. Lebensm Wiss Technol 2015; 63: 1049-55.
de Falco B, Amato M, Lanzotti V. Chia seeds products: an overview. Phytochem Rev 2017; 16: 745-60.

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Article Details

Year: 2020
Published on: 24 November, 2019
Page: [988 - 995]
Pages: 8
DOI: 10.2174/1573401315666191125105433

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