Ethyl Acetate and Aqueous Fractions of Ziziphus jujuba Prevent Acute Hypertension Induced by Angiotensin II in Rats

Author(s): Yasamin Kamkar-Del, Reza Mohebbati, Mahmoud Hosseini, Abolfazl Khajavirad, Mohammad N. Shafei*, Hassan Rakhshandeh

Journal Name: Cardiovascular & Hematological Disorders-Drug Targets
(Formerly Current Drug Targets - Cardiovascular & Hematological Disorders)

Volume 20 , Issue 2 , 2020

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Graphical Abstract:


Abstract:

Background: The effect of hydroalcoholic extract of Ziziphus jujuba (ZJ) on hypertension has been reported previously.

Objective: This experiment investigates the effect of two ethyl acetate (EA, a polar and semi-polar compound) and aqueous fractions (AQ, a polar compound) of ZJ extract on cardiovascular parameters in acute hypertension induced by angiotensin II (AngII).

Methods: Rats were randomly divided into following groups (n=7 in each group): 1) Control; 2) AngII (50 ng/kg); 3) Losartan (LOS, 30 mg/kg) + AngII; 4, 5) ethyl acetate fraction (EA150 and EA300 mg/kg) + AngII and 6, 7) aqueous fraction (AQ150 and AQ300 mg/kg) + AngII. Rats were treated with both fractions and LOS orally for four weeks and in the experiment day (28th) AngII intravenously injected and cardiovascular parameters (Systolic Blood Pressure (SBP), Mean Arterial Pressure (MAP) and Heart Rate (HR)) directly were recorded by a power lab system.

Results: AngII could significantly increase SBP and MAP (P<0.001) and decrease HR with respect to the control and these responses were attenuated by LOS. The SBP and MAP in both doses of EA+ AngII and the higher dose of AQ fractions + AngII were significantly lower than the AngII group (P<0.05 to P<0.001). Bradycardia induced by AngII was also reduced by LOS and both fractions. The comparison of two fractions also showed that the effect of EA fraction is greater than the AQ.

Conclusion: This study indicates that both fractions of the ZJ extract have a beneficial effect on hypertension. Because effect of EA was greater than AQ, we suggested that antihypertensive effects of ZJ mediated polar and nonpolar compounds.

Keywords: Ziziphus jujuba, ethyl acetate fraction, aqueous fraction, angiotensinII, hypertension, heart rate.

[1]
Chockalingam, A.; Campbell, N.R.; Fodor, J.G. Worldwide epidemic of hypertension. Can. J. Cardiol., 2006, 22(7), 553-555.
[http://dx.doi.org/10.1016/S0828-282X(06)70275-6] [PMID: 16755308]
[2]
Marc, Y.; Llorens-Cortes, C. The role of the brain renin-angiotensin system in hypertension: implications for new treatment. Prog. Neurobiol., 2011, 95(2), 89-103.
[http://dx.doi.org/10.1016/j.pneurobio.2011.06.006] [PMID: 21763394]
[3]
Moradi, M.T.; Asadi-Samani, M.; Bahmani, M. Hypotensive medicinal plants according to Ethnobotanical evidence of Iran: A Systematic Review. Int. J. Pharm. Tech. Res., 2016, 9(5), 416-426.
[4]
(a)Nguyen Dinh Cat, A.; Touyz, R.M. A new look at the renin-angiotensin system--focusing on the vascular system. Peptides, 2011, 32(10), 2141-2150.
[http://dx.doi.org/10.1016/j.peptides.2011.09.010] [PMID: 21945916]
(b)Shafei, M.N.; Faramarzi, A.; Khajavi Rad, A.; Anaeigoudari, A. Crocin prevents acute angiotensin II-induced hypertension in anesthetized rats. Avicenna J. Phytomed., 2017, 7(4), 345-352.
[PMID: 28884084]
[5]
(a)Rafieian-Kopaei, M.; Nasri, H.; Baradaran, A. Teucrium polium: Liver and kidney effects. J. Res. Med. Sci., 2014, 19(5), 478-479.
[PMID: 25097635]
(b)Rouhi-Boroujeni, H.; Asadi-Samani, M.; Moradi, M-T. A review of the medicinal plants effective on headache based on the ethnobotanical documents of Iran. Der Pharmacia Lettre, 2016, 8(3), 37-42.
[6]
Mahajan, R.T.; Chopda, M. Phyto-Pharmacology of Ziziphus jujuba Mill-A plant review. Pharmacogn. Rev., 2009, 3(6), 320.
[7]
(a)Cheng, G.; Bai, Y.; Zhao, Y.; Tao, J.; Liu, Y.; Tu, G.; Ma, L.; Liao, N.; Xu, X. Flavonoids from Ziziphus jujuba Mill var. spinosa. Tetrahedron, 2000, 56(45), 8915-8920.
[http://dx.doi.org/10.1016/S0040-4020(00)00842-5]
(b)Gao, Q-H.; Wu, C-S.; Wang, M. The jujube (Ziziphus jujuba Mill.) fruit: a review of current knowledge of fruit composition and health benefits. J. Agric. Food Chem., 2013, 61(14), 3351-3363.
[http://dx.doi.org/10.1021/jf4007032] [PMID: 23480594]
[8]
Mohebbati, R.; Bavarsad, K.; Rahimi, M.; Rakhshandeh, H.; Khajavi Rad, A.; Shafei, M.N. Protective effects of long-term administration of Ziziphus jujuba fruit extract on cardiovascular responses in L-NAME hypertensive rats. Avicenna J. Phytomed., 2018, 8(2), 143-151.
[PMID: 29632845]
[9]
Mohebbati, R.; Rahimi, M.; Bavarsad, K.; Shafei, M.N. Long-term administration of Ziziphus jujuba extract attenuates cardiovascular responses in hypertensive rats induced by angiotensinii. Anc. Sci. Life, 2017, 37(2), 68-73.
[10]
Mohebbati, R.; Iranmanesh, M.; Beheshti, F.; Mousavi, M.; Rohani, M.; Mogharrabi, M.; Soukhtanloo, M. The effect of some herbal extracts on nitric oxide production in endothelial cells 3T3 cell line. Indian J. Pharm. Sci., 2016, 12(3), 1-10.
[11]
Dolati, K.; Rakhshandeh, H.; Shafei, M.N. Effect of aqueous fraction of Rosa damascena on ileum contractile response of guinea pigs. Avicenna J. Phytomed., 2013, 3(3), 248-253.
[PMID: 25050281]
[12]
Siddiqi, H.S.; Mehmood, M.H.; Rehman, N.U.; Gilani, A.H. Studies on the antihypertensive and antidyslipidemic activities of Viola odorata leaves extract. Lipids Health Dis., 2012, 11(1), 6.
[http://dx.doi.org/10.1186/1476-511X-11-6] [PMID: 22233644]
[13]
Shafei, M.N.; Nasimi, A. Effect of glutamate stimulation of the cuneiform nucleus on cardiovascular regulation in anesthetized rats: role of the pontine Kolliker-Fuse nucleus. Brain Res., 2011, 1385, 135-143.
[http://dx.doi.org/10.1016/j.brainres.2011.02.046] [PMID: 21349254]
[14]
Vander, A.J.; Geelhoed, G.W. Inhibition of renin secretion by angiotensin. II. Proc. Soc. Exp. Biol. Med., 1965, 120(2), 399-403.
[http://dx.doi.org/10.3181/00379727-120-30547] [PMID: 4285454]
[15]
Nishi, E.E.; Bergamaschi, C.T.; Oliveira-Sales, E.B.; Simon, K.A.; Campos, R.R. Losartan reduces oxidative stress within the rostral ventrolateral medulla of rats with renovascular hypertension. Am. J. Hypertens., 2013, 26(7), 858-865.
[http://dx.doi.org/10.1093/ajh/hpt037] [PMID: 23485486]
[16]
Mozdastan, S.; Ebrahimzadeh, M.A.; Eslami, S. Effect of Increasing the Polarity of Solvent on Total Phenol and Flavonoid Contents and Antioxidant Activity of Myrtle (Myrtus communis L.). Majallah-i Danishgah-i Ulum-i Pizishki-i Mazandaran, 2015, 25(126), 68-81.
[17]
(a)Apostolidis, E.; Kwon, Y-I.; Shetty, K. Potential of cranberry-based herbal synergies for diabetes and hypertension management. Asia Pac. J. Clin. Nutr., 2006, 15(3), 433-441.
[PMID: 16837438]
(b)Oboh, G.; Ademiluyi, A.O.; Akinyemi, A.J.; Henle, T.; Saliu, J.A.; Schwarzenbolz, U. Inhibitory effect of polyphenol-rich extracts of jute leaf (Corchorus olitorius) on key enzyme linked to type 2 diabetes (α-amylase and α-glucosidase) and hypertension (angiotensin I converting) in vitro. J. Funct. Foods, 2012, 4(2), 450-458.
[http://dx.doi.org/10.1016/j.jff.2012.02.003]
[18]
Hügel, H.M.; Jackson, N.; May, B.; Zhang, A.L.; Xue, C.C. Polyphenol protection and treatment of hypertension. Phytomedicine, 2016, 23(2), 220-231.
[http://dx.doi.org/10.1016/j.phymed.2015.12.012] [PMID: 26926184]
[19]
Walter, A.; Etienne-Selloum, N.; Sarr, M.; Kane, M.O.; Beretz, A.; Schini-Kerth, V.B. Angiotensin II induces the vascular expression of VEGF and MMP-2 in vivo: preventive effect of red wine polyphenols. J. Vasc. Res., 2008, 45(5), 386-394.
[http://dx.doi.org/10.1159/000121408] [PMID: 18354258]
[20]
Du, J.; Leng, J.; Zhang, L.; Bai, G.; Yang, D.; Lin, H.; Qin, J. Angiotensin II-induced apoptosis of human umbilical vein endothelial cells was inhibited by blueberry anthocyanin through bax-and caspase 3-dependent pathways. Med. Sci. Monit., 2016, 22, 3223-3228.
[http://dx.doi.org/10.12659/MSM.896916] [PMID: 27616275]
[21]
(a)Ward, N.C.; Croft, K.D. Hypertension and oxidative stress. Clin. Exp. Pharmacol. Physiol., 2006, 33(9), 872-876.
[http://dx.doi.org/10.1111/j.1440-1681.2006.04457.x] [PMID: 16922824]
(b)Hirata, Y.; Satonaka, H. Hypertension and oxidative stress. Japan Med. Assoc. J., 2001, 44(12), 540-545.
[22]
Sarr, M.; Chataigneau, M.; Martins, S.; Schott, C.; El Bedoui, J.; Oak, M-H.; Muller, B.; Chataigneau, T.; Schini-Kerth, V.B. Red wine polyphenols prevent angiotensin II-induced hypertension and endothelial dysfunction in rats: role of NADPH oxidase. Cardiovasc. Res., 2006, 71(4), 794-802.
[http://dx.doi.org/10.1016/j.cardiores.2006.05.022] [PMID: 16822492]
[23]
Zhao, Y.; Zhang, X.; Li, J.; Bian, Y.; Sheng, M.; Liu, B.; Fu, Z.; Zhang, Y.; Yang, B.; Jujuboside, B. Jujuboside B reduces vascular tension by increasing Ca2+ influx and activating endothelial nitric oxide synthase. PLoS One, 2016, 11(2)e0149386
[http://dx.doi.org/10.1371/journal.pone.0149386] [PMID: 26901291]
[24]
Scicchitano, P.; Cameli, M.; Maiello, M.; Modesti, P.A.; Muiesan, M.L.; Novo, S.; Palmiero, P.; Saba, P.S.; Pedrinelli, R.; Ciccone, M.M. Nutraceuticals and dyslipidaemia: Beyond the common therapeutics. J. Funct. Foods, 2014, 6, 11-32.
[http://dx.doi.org/10.1016/j.jff.2013.12.006]


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Article Details

VOLUME: 20
ISSUE: 2
Year: 2020
Page: [108 - 115]
Pages: 8
DOI: 10.2174/1871529X19666191119141400
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