Background: The effect of hydroalcoholic extract of Ziziphus jujuba (ZJ) on
hypertension has been reported previously.
Objective: This experiment investigates the effect of two ethyl acetate (EA, a polar and semi-polar
compound) and aqueous fractions (AQ, a polar compound) of ZJ extract on cardiovascular
parameters in acute hypertension induced by angiotensin II (AngII).
Methods: Rats were randomly divided into following groups (n=7 in each group): 1) Control; 2)
AngII (50 ng/kg); 3) Losartan (LOS, 30 mg/kg) + AngII; 4, 5) ethyl acetate fraction (EA150 and
EA300 mg/kg) + AngII and 6, 7) aqueous fraction (AQ150 and AQ300 mg/kg) + AngII. Rats were
treated with both fractions and LOS orally for four weeks and in the experiment day (28th) AngII
intravenously injected and cardiovascular parameters (Systolic Blood Pressure (SBP), Mean
Arterial Pressure (MAP) and Heart Rate (HR)) directly were recorded by a power lab system.
Results: AngII could significantly increase SBP and MAP (P<0.001) and decrease HR with respect
to the control and these responses were attenuated by LOS. The SBP and MAP in both doses of
EA+ AngII and the higher dose of AQ fractions + AngII were significantly lower than the AngII group
(P<0.05 to P<0.001). Bradycardia induced by AngII was also reduced by LOS and both fractions.
The comparison of two fractions also showed that the effect of EA fraction is greater than the AQ.
Conclusion: This study indicates that both fractions of the ZJ extract have a beneficial effect on
hypertension. Because effect of EA was greater than AQ, we suggested that antihypertensive effects
of ZJ mediated polar and nonpolar compounds.