Background: Multi-drug resistance among pathogens is emerging due to the slow pace of
development of new antimicrobials by combinatorial chemistry. Natural products from microorganisms
obtained from under-explored habitats can be lead molecules for such discoveries. In search of
new antimicrobial compounds, Streptomyces isolate UK-201 exhibiting broad-spectrum antimicrobial
and antifungal activity, obtained from under-explored Lachhiwala Reserve forest, of the Himalayas
was selected in this study.
Objectives: The major objectives were to characterize isolate UK-201, taxonomically identify it
based on 16S rDNA sequencing and execute metabolite fingerprinting of ethyl acetate extract of
UK-201 by GC-MS.
Methods: Isolate UK-201 was characterized by phenotypic, biochemical/physiological methods and
identified by 16S rDNA sequencing. Ethyl acetate extract of this isolate exhibited antimicrobial activity
against the selected panel of gram-positive, gram-negative bacteria and fungi. The extract was
partially purified by column chromatography. Active fractions were pooled and analysed by GCMS.
The obtained compounds were tentatively identified by collated data analysis based on Similarity
Index, and observed and calculated Retention Indices.
Results: Isolate UK-201 showed 97.46% similarity to Streptomyces niveiscabiei, 96.88% to S. sasae
and S. puniciscabiei, 96.72% to S. capoamus and S. yaanensis. A low similarity percentage indicated
the taxonomic novelty of the isolate and was confirmed by comparing it with phenotypic characteristics
with the nearest matches. Metabolite fingerprinting showed the presence of twenty-four novel
Conclusion: This study showed that bioprospection from under-explored habitats conferred novel
bio and chemodiversity.