Background: A simple quantification technique by liquid chromatography electrospray ionization-
tandem mass spectrometry (LC-ESI-MS/MS) is required for regorafenib in biological matrices
with bioavailability studies in healthy rabbits, when compared with reported techniques.
Objective: The main aim of the research work was to develop a validated LC-ESI-MS/MS technique
for the quantification of regorafenib and application to bioavailability studies in healthy rabbits.
Methods: Chromatographic separation was achieved with hypersil-C18 analytical column (50mm×4.6 mm,
4μm) and mobile phase composition of acetonitrile and 5mM ammonium acetate in the proportion of
70:30. The mobile phase was infused into the column with high pressure to get a 0.7 ml/min flow rate.
The total retention time of the analyte is promising when compared with the existed methods for
regorafenib. Quantitation was processed by monitoring transitions of m/z -483.0/262.0 and 450.0/260.0
for regorafenib and internal standard respectively in multiple reaction monitoring.
Results: The linearity equation and correlation coefficient (R2) findings were y =0.9948x+2.6624 and
0.998 respectively. The intra and inter-day precision of the developed technique was found between
1.00 – 8.50% for the QC-samples (2, 4, 240 and 480ng/ml). From bioavailability study, the drug was
shown Tmax of 3.688 ± 0.754; average AUC0→α and AUC0→t were 6476.81 ± 259.59 and 6213.845 ±
257.892 respectively and Cmax was found to be 676.91 ± 22.045 in healthy rabbits.
Conclusion: The developed technique was validated and successfully applied in the pharmacokinetic
study of the drug (40 mg tablet) administered through the oral route in healthy rabbits.