Background: Silver nanoparticles synthesized by the bio-green method have been applied
to various biomedical applications. These procedures are simple, eco-friendly and serve as an
alternative to complex chemical methods for the preparation of nanomaterials.
Objective: In the present study, phytosynthesis of silver nanoparticles, to examine their antioxidant
potential, toxic effects towards bacterial-, fungal-strains, brine shrimp nauplii and cancer cells was
Methods: Methanolic extract of Euphorbia wallichii roots was used for the synthesis of silver
nanoparticles. The synthesis was monitored and confirmed by UV-visible spectroscopy, Fourier
Transform Infra-Red (FTIR) spectrometric analysis, Field Emission Scanning Electron Microscope
(FESEM), Energy Dispersive X-ray (EDX) and X-Ray Powder Diffraction (XRD).
Results: The synthesized particles were average 63±8 nm in size. Involvement of phenolic
(46.7±2.4 µg GAE/mg) and flavonoid (11.7±1.2 µg QE/mg) compounds as capping agents was
also measured. Nanoparticles showed antioxidant properties in terms of free radical scavenging
potential (59.63±1.0 %), reducing power (44.52±1.34 µg AAE/mg) and total antioxidant capacity
(60.48±2.2 µg AAE/mg). The nanoparticles showed potent cytotoxic effects against brine shrimp
nauplii (LD50 66.83 µg/ml), proliferation and cell death of HeLa cells as determined by MTT
(LD50 0.3923 µg/ml) and TUNEL assays, respectively. Antimicrobial results revealed that silver
nanoparticles were found to be more potent against pathogenic fungal (maximum active against A.
fumigatus, MIC 15 µg/disc) and bacterial strains (maximum active against S. aureus, MIC 3.33
μg/disc) than the E. wallichii extract alone.
Conclusion: These results support the advantages of using an eco-friendly and cost-effective
method for synthesis of nanoparticles with antioxidant, cytotoxic and antimicrobial potential.