Background: The current research aimed to explore secondary metabolites and antibacterial
activity against bacterial agents associated with bovine mastitis from extracts prepared from the
peel of fruits of Punica granatum L.. Mastitis is an inflammation in the mammary gland mainly due
to infection of bacteria and can be presented clinically or subclinically. The infectious bovine mastitis
disease is associated with the reduction and changes in the composition of the milk, as well as in
the early disposal of the animal.
Objective: We describe the bactericidal activity of ethanolic extracts of the fruit peel of P. granatum,
against etiological agents of bovine mastitis (Escherichia coli, Staphylococcus aureus and Streptococcus
Method: The bactericidal activity was evaluated according to the standards of the European Committee
for Antimicrobial Susceptibility Testing and by the broth microdilution methodology according
to the standards of the Clinical and Laboratory Standards Institute. The fruit peel extracts of P.
granatum were prepared with ethanol solvent (90%).
Results: The best results of total inhibition of bacterial growth of the three strains tested were obtained
with the concentrations of 75mg/mL of the extract after 1 and 3 hours of treatment. Cell
membrane integrity analysis by flow cytometry showed that 1 h of contact there was loss of integrity
of the wall and cell membranes.
Conclusion: We can suggest that the ethanolic extract of the fruit peel of P. granatum can be an important
natural coadjutant compound in the treatment of mastitis as well as being used in the formulation