Phytochemical and Cytotoxicity Studies of Callistemon viminalis Leaves Extract Growing in Egypt

Author(s): Amal H. Ahmed*

Journal Name: Current Pharmaceutical Biotechnology

Volume 21 , Issue 3 , 2020

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Graphical Abstract:


Objective: To isolate and identify new compounds from the methanolic extract of Callistemon viminalis leaves collected in Cairo, Egypt and evaluate its cytotoxic and hepatoprotective potentials.

Methods: The methanolic extract of Callistemon viminalis leaves was fractionated and subjected to different chromatographic techniques to isolate pure, new compounds which were identified by nuclear magnetic resonance (NMR), spectroscopic analysis and mass spectrometric methods. The methanolic extract of the leaves was assessed for its cytotoxic and hepatoprotective activities against Hepatocellular carcinoma cells (Hep G-2 cell line) by estimating the viability of the HepG2 cells by the MTT reduction assay.

Results: Six compounds were isolated and identified for the first time from the methanolic extract of Callistemon viminalis leaves, three compounds are new flavonoids namely; 3-O-[α-L-arabinopyranosyl- (1→2)-α-L-arabinopyranosyl)]-3′-O-methylquercetin (C1); 5,7,3',4' tetrahydroxy isoflavone-7-O-α- L-1C4- rhamnopyranosyl (1'''-6'')-O-β-D-4C1-glucopyranoside (C2) and 6-methyl-5,7-dihydroxy-4'- methoxyflavone (C6) along with the three known ones; hyperoside (C3), rutin (C4) and isoquercitrin (C5).

Conclusion: The methanolic extract of the leaves showed strong cytotoxic activity against Hepatocellular carcinoma cells (Hep G-2 cell line) and weak hepatoprotective effect.

Keywords: Flavonoid, glycosides, Callistemon viminalis, cytotoxic, hepatoprotective activity, leaves extract, hepatocellular carcinoma.

Edward, F.; Watson, D.G. Callistemon viminalis, - Mature Weeping Bottlebrush. Fact Sheet ST. 1993.
Solomon, C.; Arukwe, I.; Onuoha, I. Preliminary phytochemical screening of different solvent extracts of stem bark and roots of Dennetia tripetala. Res. Library, 2013, 3(3), 10-13.
Srivastava, S.; Ahmad, A.; Syamsunder, K.; Aggarwal, K.; Khanuja, S.J. Essential oil composition of Callistemon viminalis leaves from India. Flavour Frag., 2003, 18(5), 363-361.
Oyedeji, O.O.; Lawal, O.A.; Shode, F.O.; Oyedeji, A.O. Chemical composition and antibacterial activity of the essential oils of Callistemon citrinus and Callistemon viminalis from South Africa. Molecules, 2009, 14(6), 1990-1998.
[] [PMID: 19513000]
Abdelhady, M.I.; Motaal, A.A.; Beerhues, L.; Beerhues, L. Total phenolic content and antioxidant activity of standardized extracts from leaves and cell cultures of three callistemon species. Am. J. Plant Sci., 2011, 2, 847-850.
Kanjilal, P.C.; Das, A. Flora of Assam; Omsons Publications: New Delhi, 1992.
Mahmoud, I.I.; Moharram, F.A.; Marzouk, M.S.; Linscheid, M.W.; Saleh, M.I. Polyphenolic constituents of Callistemon lanceolatus leaves. Pharmazie, 2002, 57(7), 494-496.
[PMID: 12168536]
Abdelhady, M.I.; Amel, M.K.; Madiha, M.R.; Tawfik, N.F.; Abdelkhalik, S.M. Antinociceptive and antischistosomal activities of aqueous methanolic extract of Callistemon viridiflorus leaves in addition of detection of new polyphenolic metabolite. J. Pharm. Res., 2012, 5(7), 3777-3783.
Abdelhady, M.I.S.; Kamal, A.M.; Tawfik, N.F.; Abdelkhalik, S.M. Polyphenolic constituents of the methanolic extract of Callistemon viridiflorous leaves and its antimicrobial activity. Pharmacogn. J., 2012, 4(31), 47-53.
Salem, M.Z.M.; El-Hefny, M.; Nasser, R.A.; Ali, H.M.; El-Shanhorey, N.A.; Elansary, H.O. Medicinal and biological values of Callistemon viminalis extracts: History, current situation and prospects. Asian Pac. J. Trop. Med., 2017, 10(3), 229-237.[]
[PMID: 28442106]
Ou, S.; Kwok, K.; Li, Y.; Fu, L. In vitro study of possible role of dietary fiber in lowering postprandial serum glucose. J. Agric. Food Chem., 2001, 49(2), 1026-1029.
[] [PMID: 11262066]
Rhabasa-Lhoret, R.; Chiasson, J.L. International textbook of Diabetes mellitus; 3rd Edition, UK: John Wiley and Sons Ltd.; . , 2004, 1, pp. 901-914.
Sharma, R.K.; Kotoky, R.; Bhattacharya, P.R. Volatile oil from the leaves of Callistemon lanceolatus D.C. grown in north-eastern India. Flavour Fragrance J., 2006, 21, 239-240.
Mabry, T.J.; Markham, K.R.; Thomas, M.B. The systematic identification of flavonoids; Switzerland: Springer Nature. , 1970.
Sudhankar, M.; Rao, Ch.V.; Lakshman, A.R.; Raju, D.B. Pharmacognostic study and anti-inflammatory activity of Callistemon lanceolatus leaf. Acta Pharm. Turc., 2004, 46, 131-139.
Mosmann, T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods, 1983, 65(1-2), 55-63.
[] [PMID: 6606682]
Gomha, S.M.; Riyadh, S.M.; Mahmmoud, E.A.; Elaasser, M.M. Synthesis and anticancer activities of thiazoles, 1,3-thiazines, and thiazolidine using chitosan-grafted-poly(vinylpyridine) as basic catalyst. Heterocycles, 2015, 91(6), 1227-1243.
Thirunavukkarasu, P.; Asha, S.; Ramanathan, T.; Balasubramanian, T.; Shanmogapriya, R.; Renugadevi, G. In vitro hepatoprotective activity of isolated fractions of Cressa cretica. Pharm. Chem. J., 2014, 48(2), 121-126.
Ke, H.; Hisayoshi, K.; Aijun, D.; Yongkui Jing, Y.; Iwasaki, S.; Yao, X. Antineoplastic agents III: Steroidal glycosides from Solanum nigrum. Planta Med., 1999, 65, 35.
[] [PMID: 10083842]
Kamal, A.; Bharathi, V.E.; Ramaiah, J.M.; Dastagiri, D.; Reddy, S.J.; Viswanath, A.; Sultana, F.; Pushpavalli, S.N.C.V.L.; Pal-Bhadra, M.; Srivastava, H.K.; Sastry, N.G.; Juvekar, A.; Sen, S.; Zingde, S. Quinazolinone linked pyrrolo[2,1-c][1,4]benzodiazepine (PBD) conjugates: Design, synthesis and biological evaluation as potential anticancer agents. Bioorg. Med. Chem., 2010, 18(2), 526-542.
[] [PMID: 20031423]
Alaniya, M.D. Kavtaradze NSh N Sh; Bassarello C, Skhirtladze AV, Pizza C, Kutateladze I, Flavonoid glycosides from Astragalus galegiformis leaves. Chem. Nat. Compd., 2006, 42(6), 681-685.
Shaker, K.H.; Al Jubiri, S.M. Abd El-hady, F.K.; Al-Sehemi. A.G. New compounds from Bassia muricata and Fagonia indica. Int. J. Pharm. Sci. Rev. Res., 2013, 23(1), 231-236.
Marzouk, M.S.; Magda, I.T.; Omaima, D. El-Gindi, Marwa S. Abou Bakr, Isoflavonoid glycosides and rotenoids from Pongamia pinnata leaves. Z. Naturforsch, 2008, 63c, 1-7.
Cho, J.Y.; Moon, S.H.; Lee, K.H.; Park, K.H. A novel benzoyl glucoside and phenolic compounds from the leaves of Camellia japonica. Food Sci. Biotechnol., 2008, 17, 1060-1065.
Sukito, A.; Tachibana, S. Isolation of hyperoside and isoquercitrin from Camellia sasanqua as antioxidant agents. Pak. J. Biol. Sci., 2014, 17(8), 999-1006.
[] [PMID: 26031018]
Markham, K.R. Techniques of flavonoid identification; Biological Techniques Series; Academic Press: England, 1981, pp. 36-51.

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Article Details

Year: 2020
Published on: 16 March, 2020
Page: [219 - 225]
Pages: 7
DOI: 10.2174/1389201020666191107110341
Price: $65

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