Background: The cortex of Mallotus japonicus (Euphorbiaceae) has traditionally been
used to treat gastric ulcers, duodenal ulcers, and gastric hyperacidity in Japan. A large number of
studies have recently focused on its effects on Inflammatory Bowel Disease (IBD).
Objective: The aim of the present study was to examine the effects of M. japonicus (MJ) extracts on
large intestinal diarrhea and inflammation using Inflammatory Bowel Disease (IBD) mouse models.
Methods: The present study used 3% Dextran Sulfate Sodium (DSS)-treated colitis models. Red
blood cell, platelet, and leukocyte counts in addition to hematocrit (Ht), hemoglobin (Hb), and colonic
cytokine and chemokine levels were measured in DSS-treated C57BL/6J mice during the experimental
Results: The Disease Activity Index (DAI) was lower in 3% DSS-treated mice orally administered
MJ (200 and 500 mg/kg) than in mice administered 3% DSS only. Furthermore, MJ inhibited decreases
in red blood cell and platelet counts as well as Hb and Ht levels in DSS-treated mice. Colon
histology using direct fast scarlet staining revealed that MJ prevented mucosal membrane ulceration
and eosinophil infiltration of the mucosal membrane induced by the DSS treatment. Increases in
colonic Monocyte Chemoattractant Protein 1 (MCP)-1, interleukin (IL)-1β, and Tumor Necrosis
Factor (TNF)-α levels in DSS-treated mice were reduced by orally administered MJ extracts.
Conclusion: The present results suggest that M. japonicus cortex extracts are an effective treatment
for IBD through the inhibition of increases in colonic IL-1β, TNF-α, and MCP-1 levels and eosinophil
infiltration of the colon in DSS-treated mice.