An Assessment of the Effects of Azodicarbonamide-containing Diet on Neurobehaviour, Brain Antioxidant Status and Membrane Lipid Peroxidation Status in Rats

Author(s): Anthony T. Olofinnade, Adegboyega Adeyeba, Adejoke Y. Onaolapo, Olakunle J. Onaolapo*

Journal Name: Central Nervous System Agents in Medicinal Chemistry
(Formerly Current Medicinal Chemistry - Central Nervous System Agents)

Volume 20 , Issue 1 , 2020

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Graphical Abstract:


Abstract:

Background: Azodicarbonamide is a dough-enhancer used in the process of breadmaking in countries like Nigeria. While there have been suggestions that it is a sensitizer of the respiratory system, there is a dearth of information on its effects on the central nervous system.

Aim: This study assessed the effects of azodicarbonamide on the central nervous system (ADA) in rats.

Objective: The effects of ADA-containing diet on neurobehaviour, brain antioxidant status, and neuromorphology of selected brain regions in rats were examined.

Methods: Forty adult rats were randomly-assigned into four groups of ten rats each, and were given standard diet or diet containing ADA at 1, 2 and 4% respectively. Rats were fed a standard diet or ADA-containing diet for a period of 28 days. Weekly body weight assessment and daily estimation of food intake were done. Behavioural tests {in the Open field, Y-maze, radial-arm maze, and Elevated Plus Maze (EPM)} were conducted on day 29. Twenty-four hours after the last behavioural test, animals were euthanised, whole brains were dissected, weighed, and either homogenised for assessment of lipid peroxidation and antioxidant status; or sectioned and processed for general histology.

Results: Consumption of ADA-containing diet was associated with a significant decrease in weight gain/food intake, and significant suppression of horizontal locomotion and rearing behaviours; however, grooming activity increased significantly. Also, there was a significant reduction of open-arm time in the EPM and a significant increase in Y-maze alternation (at the lowest concentration of ADA). ADA-containing diet was not associated with significant changes in brain oxidative status or neuromorphology.

Conclusion: The study showed that while ADA-containing diet may alter neurobehaviour in rats; this was not associated with evidence of brain oxidative stress or neuro-histomorphological alterations.

Keywords: Dough enhancer, food additive, neurobehaviour, oxidative stress, neuromorphology, central inhibition.

[1]
WHO World health Organisation, Food additives fact sheet. 2017.
[2]
Onaolapo, A.Y.; Onaolapo, O.J. Food additives, food and the concept of ‘food addiction’: Is stimulation of the brain reward circuit by food sufficient to trigger addiction? Pathophysiology, 2018, 25(4), 263-276.
[http://dx.doi.org/10.1016/j.pathophys.2018.04.002] [PMID: 29673924]
[3]
Omojokun, J. Regulation and Enforcement of Legislation on Food Safety.In: Nigeria, Mycotoxin and Food Safety in Developing Countries; Hussaini, A.M., Ed.; Intech Open: UK, 2013.
[http://dx.doi.org/10.5772/54423.7/47]
[4]
Li, M.; Zhu, K-X.; Guo, X-G.; Brijs, K.; Zhou, H-M. Natural additives in wheat‐based pasta and noodle products: Opportunities for enhanced nutritional and functional properties. Compr. Rev. Food Sci. Food Saf., 2014, 13, 347-357.
[http://dx.doi.org/10.1111/1541-4337.12066]
[5]
Landau, E. Subway to remove ‘dough conditioner’ chemical from bread 2014.Available from. https://edition.cnn.com/2014/02/06/health/subway-bread-chemical/index.html
[6]
UK Occupational Health and Safety. Substances causing/worsening asthma, 2013.
[7]
Kimmerle, G. Toxicological studies on Porofor ADC, Urazol and plastic foam extracts. Unpublished report, Institute of Toxicology, Bayer AG, Wuppertal-Elberfeld. 1965.
[8]
Gafford, F.H.; Sharry, P.M.; Pittman, J.A., Jr Effect of azodicarbonamide (1,1′-azobisformamide) on thyroid function. J. Clin. Endocrinol. Metab., 1971, 32(5), 659-662.
[http://dx.doi.org/10.1210/jcem-32-5-659] [PMID: 4102820]
[9]
Ash, M.; Ash, I. Handbook of green chemicals, 2nd ed; , 2004.
[10]
Sahi, S.S. Ascorbic acid and redox agents in bakery systems.In: Bakery Products Science and Technology, 2nd ed; Wiley Online Library: New Jersey, USA, 2014, pp. 183-197.
[http://dx.doi.org/10.1002/9781118792001.ch10]
[11]
Onaolapo, A.Y.; Onaolapo, O.J.; Nwoha, P.U. Aspartame and the hippocampus: Revealing a bi-directional, dose/time-dependent behavioural and morphological shift in mice. Neurobiol. Learn. Mem., 2017, 139, 76-88.
[http://dx.doi.org/10.1016/j.nlm.2016.12.021] [PMID: 28049023]
[12]
Onaolapo, A.Y.; Onaolapo, O.J.; Nwoha, P.U. Methyl aspartylphenylalanine, the pons and cerebellum in mice: An evaluation of motor, morphological, biochemical, immunohistochemical and apoptotic effects. J. Chem. Neuroanat., 2017, 86, 67-77.
[http://dx.doi.org/10.1016/j.jchemneu.2017.09.001] [PMID: 28890110]
[13]
Onaolapo, O.J.; Adekola, M.A.; Azeez, T.O.; Salami, K.; Onaolapo, A.Y. l-Methionine and silymarin: A comparison of prophylactic protective capabilities in acetaminophen-induced injuries of the liver, kidney and cerebral cortex. Biomed. Pharmacother., 2017, 85, 323-333.
[http://dx.doi.org/10.1016/j.biopha.2016.11.033] [PMID: 27889232]
[14]
Onaolapo, A.Y.; Aina, O.A.; Onaolapo, O.J. Melatonin attenuates behavioural deficits and reduces brain oxidative stress in a rodent model of schizophrenia. Biomed. Pharmacother., 2017, 92, 373-383.
[http://dx.doi.org/10.1016/j.biopha.2017.05.094] [PMID: 28554133]
[15]
Onaolapo, O.J.; Onaolapo, A.Y.; Akanmu, M.A.; Olayiwola, G. Changes in spontaneous working-memory, memory-recall and approach-avoidance following “low dose” monosodium glutamate in mice. AIMS Neurosci., 2016a, 3, 317-337.
[http://dx.doi.org/10.3934/Neuroscience.2016.3.317]
[16]
Onaolapo, O.J.; Ayanwale, T.; Agoi, O.; Adetimehin, C.; Onaolapo, A.Y. Zinc tempers haloperidol-induced behavioural changes in healthy mice. Int. J. Neurosci. Behavioral Sci., 2016, 4, 21-31.
[http://dx.doi.org/10.13189/ijnbs.2016.040201]
[17]
Onaolapo, A.Y.; Onaolapo, O.J. Nevirapine mitigates monosodium glutamate induced neurotoxicity and oxidative stress changes in prepubertal mice. Ann. Med. Res., 2018, 25, 518-524.
[http://dx.doi.org/10.5455/annalsmedres.2018.06.118]
[18]
Onaolapo, A.Y.; Adebayo, A.A.; Onaolapo, O.J. Oral phenytoin protects against experimental cyclophosphamide-chemotherapy induced hair loss. Pathophysiology, 2018, 25, 31-39.
[19]
Onaolapo, A.Y.; Ayeni, O.J.; Ogundeji, M.O.; Ajao, A.; Onaolapo, O.J.; Owolabi, A.R. Subchronic ketamine alters behaviour, metabolic indices and brain morphology in adolescent rats: Involvement of oxidative stress, glutamate toxicity and caspase-3-mediated apoptosis. J. Chem. Neuroanat., 2019, 96, 22-33.
[http://dx.doi.org/10.1016/j.jchemneu.2018.12.002] [PMID: 30529750]
[20]
Onaolapo, A.Y.; Onaolapo, O.J.; Nwoha, P.U. Alterations in behaviour, cerebral cortical morphology and cerebral oxidative stress markers following aspartame ingestion. J. Chem. Neuroanat., 2016, 78, 42-56.
[http://dx.doi.org/10.1016/j.jchemneu.2016.08.006] [PMID: 27565676]
[21]
Oser, B.L.; Oser, M.; Morgareidge, K.; Sternberg, S.S. Studies of the safety of azodicarbonamide as a flour maturing agent. Toxicol. Appl. Pharmacol., 1965, 7, 445-472.
[http://dx.doi.org/10.1016/0041-008X(65)90146-8] [PMID: 14288817]
[22]
Medinsky, M.A.; Bechtold, W.E.; Birnbaum, L.S.; Bond, J.A.; Burt, D.G.; Cheng, Y.S.; Gillett, N.A.; Gulati, D.K.; Hobbs, C.H.; Pickrell, J.A. Effect of inhaled azodicarbonamide on F344/N rats and B6C3F1 mice with 2-week and 13-week inhalation exposures. Fundam. Appl. Toxicol., 1990, 15(2), 308-319.
[http://dx.doi.org/10.1016/0272-0590(90)90057-Q] [PMID: 2227158]
[23]
Hall, W.G.; Bryan, T.E. The ontogeny of feeding in rats: IV. Taste development as measured by intake and behavioral responses to oral infusions of sucrose and quinine. J. Comp. Physiol. Psychol., 1981, 95(2), 240-251.
[http://dx.doi.org/10.1037/h0077771] [PMID: 7229159]
[24]
Nachman, M. Taste preferences for sodium salts by adrenalectomized rats. J. Comp. Physiol. Psychol., 1962, 55, 1124-1129.
[http://dx.doi.org/10.1037/h0041348] [PMID: 13937025]
[25]
Maranghi, F.; Tassinari, R.; Lagatta, V.; Moracci, G.; Macrì, C.; Eusepi, A.; Di Virgilio, A.; Scattoni, M.L.; Calamandrei, G. Effects of the food contaminant semicarbazide following oral administration in juvenile Sprague-Dawley rats. Food Chem. Toxicol., 2009, 47(2), 472-479.
[http://dx.doi.org/10.1016/j.fct.2008.12.003] [PMID: 19110027]
[26]
Degroot, A.; Parent, M.B. Increasing acetylcholine levels in the hippocampus or entorhinal cortex reverses the impairing effects of septal GABA receptor activation on spontaneous alternation. Learn. Mem., 2000, 7(5), 293-302.
[http://dx.doi.org/10.1101/lm.32200] [PMID: 11040261]


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Article Details

VOLUME: 20
ISSUE: 1
Year: 2020
Page: [49 - 57]
Pages: 9
DOI: 10.2174/1871524919666191104154009

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