Background: Kuding tea, a Traditional Chinese drink, has a history of thousands of years
in China. Triterpenoid saponins in Kuding tea are regarded as one of the major functional ingredients.
Objective: The aim of this paper was to establish separation progress for the isolation and purification
of five triterpenoid saponins (kudinoside A, C, D, F, G) from Kuding tea.
Methods: Nine types of resins, including seven macroporous resins and two MCI-GEL resins, were
firstly used for purifying triterpenoid saponins by the adsorption and desorption tests. Further dynamic
adsorption/desorption experiments were carried out to obtain the optimal parameters for the five
targeted saponins. Then the purification of five triterpenoid saponins (kudinoside A, C, D, F, G) was
completed by semi-preparative high-performance liquid chromatography (semi-pHPLC).
Results: As of optimized results, the HP20SS MCI-GEL was selected as the optimal one. The data
also showed that 65.24 mg of refined extract including 7.04 mg kudinoside A, 3.52 mg kudinoside C,
4.04 mg kudinoside D, 4.13 mg kudinoside F, and 34.45 mg kudinoside G, could be isolated and purified
from 645.90 mg of crude extract in which the content of five saponins was 81.51% and the average
recovery reached 69.76%. The final contents of five saponins increased 6.91-fold as compared
to the crude extract.
Conclusion: The established separation progress was highly efficient, making it a potential approach
for the large-scale production in the laboratory and providing several markers of triterpenoid saponins
for quality control of Kuding tea or its processing products.