Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications

Cutting Edge Techniques in Biophysics, Biochemistry and Cell Biology: From Principle to Applications

Advances in biomedical research have had a profound effect on human health outcomes over the last century. Biophysical, biochemical and cellular techniques are now the backbone of modern biomedical ...
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Animal Cell Culture: From Fundamental Techniques to Biomedical Applications

Pp. 1-14 (14)

Neetu Mishra and Joyita Banerjee

Abstract

Animal cell culture techniques in today’s scenario have become an indispensable tool in the field of biomedical research. It provides a basis to study molecular and biochemical changes associated with disease pathogenesis. It explicitly provides a scope to study gene expressions, regulation, proliferation, and differentiation in normal as well as pathologic conditions. The culturing of animal cells requires aseptic conditions and vital technical skills to carry out successful cell culture experiments. It provides an appropriate model for studying cell and molecular biology, biochemical changes in cells, drug screening and efficacy etc. This chapter describes the essential techniques of animal cell culture as well as its applications.

Keywords:

Animal cell culture, Adherent cells, Bacterial contamination, Biomedical research, Biomedical applications, Cell counting, Cell lines, Cell viability, Contaminants, Cross-contamination, Cryopreservation, Laboratory instruments, Mycoplasma contamination, MTT assay, Primary cell cultures, Secondary cell cultures, Suspension cells, Thawing of cells, Virus contamination, Yeast contamination.

Affiliation:

Symbiosis School of Biological Sciences (Formerly called Symbiosis School of Biomedical Sciences), Symbiosis International (Deemed University), Lavale, Pune, Maharashtra, India.