Title:The Benefit of Passion Fruit as an Anti-ulcerogenic Diet: Scientific Evidence by In vitro and In silico H+/K+ATPase Inhibitory Activity Assessment
VOLUME: 16 ISSUE: 5
Author(s):Vineeth A. Kumar, Mridula Ramkumar and S.K. Kanthlal*
Affiliation:Department of Pharmacology, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, AIMS Health Science Campus, Kochi, Department of Pharmacology, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, AIMS Health Science Campus, Kochi, Department of Pharmacology, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham, AIMS Health Science Campus, Kochi
Keywords:Passion fruit, ulcer, H+/K+ ATPase, flavonoids, oxidation, glide program.
Abstract:
Background: H+/K+ ATPase a protein present in the gastric parietal cells is a better target
for the prevention and treatment of gastric ulcer. Plant flavonoids have been reported to elicit
anti-ulcer activity by inhibiting the proton pump as well as by antioxidant defense mechanism.
Methods: Chloroform fraction of hydro-alcoholic extract of passion fruit was screened for proton
pump inhibitory assay using goat parietal cell. In-silico computational docking studies were carried
out using Glide program in order to validate the inhibitory action of selected constituents.
Results: The flavonoid rich fruit possess a promising radical scavenging activity against DPPH.
10.41μg/mL is sufficient to inhibit 50% of ATPase enzyme activity. A synergistic activity was also
achieved by the fruit with sub-effective doses of lansoprazole. Fenton's oxidation induced by H2O2
was also blunted by the fruit extract.
Conclusion: The in-vitro and in-silico findings indicated that, passion fruit can be a good dietary
supplement for the prevention and management of ulcer.
