A Neuroinflammation Inhibitor, Hypoxylon xanthone A, from Soil Fungus Hypoxylon sp.

Author(s): Ying Xiao, Bing Xu, You Kang, Yuedi Li, Yong Cui*, Wei Liu, Zheng Xiang*

Journal Name: Letters in Organic Chemistry

Volume 17 , Issue 2 , 2020

Become EABM
Become Reviewer
Call for Editor

Graphical Abstract:


Hypoxylon sp. was used to ferment at 25°C for 45 days. The solid culture of Hypoxylon sp. was extracted with 75% EtOH under ultrasonic for twice. And the dried combined extracts were then suspended in H2O and partitioned with ethyl acetate. EtOAc extracts were subjected to a silica gel column and eluted with petroleum ether - acetone to afford seven fractions. Sephadex LH-20 and RPHPLC were used subsequently to yield a novel xanthone metabolite (Hypoxylon xanthone A). Its structure was elucidated based on HR-ESI-MS, 1D-, 2D-NMR spectra, and the comparison of the experimental and calculated ECD spectra. The anti-neuroinflammatory assay of Hypoxylon xanthone A, as manifested by the inhibitory effect on LPS-induced NO production in BV-2 microglial cells, indicated almost the same inhibitory effect as minocycline in a dose-dependent manner within the concentration of 1-50 μM, suggesting that Hypoxylon xanthone A could be a new potential neuroinflammation inhibitor.

Keywords: Soil fungus Hypoxylon sp., metabolite, NMR spectrum, ECD, chemical constituent, anti-neuroinflammatory.

Li, J.; Jiang, Z.; Li, X.; Hou, Y.; Liu, F.; Li, N.; Liu, X.; Yang, L.; Chen, G. Bioorg. Med. Chem. Lett., 2015, 25(1), 53-58.
[http://dx.doi.org/10.1016/j.bmcl.2014.11.015] [PMID: 25466192]
Martin, S.; Kreisl, W.C. Curr. Neurol. Neurosci. Rep., 2017, 17, 25-36.
[http://dx.doi.org/10.1007/s11910-017-0733-2] [PMID: 28283959]
Valeria, C.; Edison, P. Alzheimers Dement., 2016, 12, 719-732.
[http://dx.doi.org/10.1016/j.jalz.2016.02.010] [PMID: 27179961]
Wei, Y.; Sui, D.J.; Xu, H.M.; Ouuang, Z.; Wu, N.; Wang, D.J.; Zhang, X.Y.; Qian, D.W. Chin. J. Nat. Med., 2017, 15, 905-911.
[PMID: 29329647]
Yu, W.G.; Shen, Y.; Wu, J.Z.; Gao, Y.B.; Zhang, L.X. Chin. J. Nat. Med., 2018, 16(5), 330-338.
[http://dx.doi.org/10.1016/S1875-5364(18)30064-5] [PMID: 29860993]
Wei, Z.; Ji, Y.B.; Li, W.L.; Dong, J.; Chen, N.; Yu, X.F.; Wu, J.; Zhao, D.; Xiang, Z. J. Asian Nat. Prod. Res., 2017, 19, 993-999.
[http://dx.doi.org/10.1080/10286020.2016.1275584] [PMID: 28671852]
Ahmad, B.; Rizwan, M.; Rauf, A.; Raza, M.; Azam, S.; Bashir, S.; Molnar, J.; Csonka, A.; Szabo, D. Asian Pac. J. Cancer Prev., 2016, 17(4), 2083-2087.
[http://dx.doi.org/10.7314/APJCP.2016.17.4.2083] [PMID: 27221899]
Kaur, T.; Kaur, A.; Sharma, V.; Manhas, R.K. Front. Microbiol., 2016, 7, 1004-1013.
[http://dx.doi.org/10.3389/fmicb.2016.01004] [PMID: 27446043]
Shi, H.X.; Feng, B.M.; Chen, G.; Bai, J.; Hua, H.M.; Zhao, D.; Wang, H.F.; Pei, Y.H. Magn. Reson. Chem., 2016, 54(8), 674-676.
[http://dx.doi.org/10.1002/mrc.4429] [PMID: 27028453]
Tomsheck, A.R.; Strobel, G.A.; Booth, E.; Geary, B.; Spakowicz, D.; Knighton, B.; Floerchinger, C.; Sears, J.; Liarzi, O.; Ezra, D. Microb. Ecol., 2010, 60(4), 903-914.
[http://dx.doi.org/10.1007/s00248-010-9759-6] [PMID: 20953951]
Liang, H.Q.; Zhang, D.W.; Guo, S.X.; Yu, J. J. Asian Nat. Prod. Res., 2018, 20(10), 951-956.
[http://dx.doi.org/10.1080/10286020.2018.1485662] [PMID: 30001634]
Shaw, J.J.; Berbasova, T.; Sasaki, T.; Jefferson-George, K.; Spakowicz, D.J.; Dunican, B.F.; Portero, C.E.; Narváez-Trujillo, A.; Strobel, S.A. J. Biol. Chem., 2015, 290(13), 8511-8526.
[http://dx.doi.org/10.1074/jbc.M114.636159] [PMID: 25648891]
Shagufta; Irshad, A. Eur. J. Med. Chem., 2016, 116, 267-280.
Hou, Y.; Wu, C.F.; Yang, J.Y.; He, X.; Bi, X.L.; Yu, L.; Guo, T. Prog. Neuropsychopharmacol. Biol. Psychiatry, 2006, 30(8), 1523-1528.
[http://dx.doi.org/10.1016/j.pnpbp.2006.05.006] [PMID: 16806626]

Rights & PermissionsPrintExport Cite as

Article Details

Year: 2020
Published on: 07 January, 2020
Page: [116 - 120]
Pages: 5
DOI: 10.2174/1570178616666191023100252
Price: $65

Article Metrics

PDF: 22