Pharmacokinetics of Picroside I, II, III, IV in Rat Plasma by UPLCMS/ MS

Author(s): Haili Xie, Xiaojie Lu, Weiqiang Jin, Hua Zhou, Dongxin Chen, Xianqin Wang*, Yunfang Zhou*

Journal Name: Current Pharmaceutical Analysis

Volume 16 , Issue 4 , 2020

Become EABM
Become Reviewer

Graphical Abstract:


Abstract:

Background: Modern pharmacological studies show that rhizoma coptidis has protective effects on the liver, gallbladder, kidney, cerebral ischemia-reperfusion, local hypoxia injury, antiinflammatory, bone injury, nerve cells and myocardial cells. The effective components have been isolated from picroside I, II, III and IV.

Introduction: A selective and sensitive ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) method was developed for the simultaneous quantitative determination of picroside I, II, III and IV in rat plasma to aid the pharmacokinetics studies.

Methods: Sprague-Dawley (SD) rats were orally administered with 10 mg/kg, intravenously injected with 1 mg/kg for the mixture of picroside I, II, III and IV. The biological samples were collected at 0.083 3 h, 0.25 h, 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 24 h. A UPLC BEH C18 column (2.1 mm×50 mm, 1.7 μm) was used for chromatographic separation with the mobile phase consisting of acetonitrile and 0.1% formic acid by gradient elution. The flow rate was 0.4 mL/min. Multiple reaction monitoring (MRM) transitions were m/z 491.1→147.1 for picroside I, m/z 511.1→234.9 for picroside II, m/z 537.3→174.8 for picroside III and m/z 507.3→163.1 for picroside IV in negative ion mode.

Results: The inter-day precision was less than 13%, the intra-day precision was less than 15%. The accuracy ranged from 89.4% to 111.1%. Recovery was higher than 79.1%, and the matrix effect ranged from 96.2% to 109.0%.

Conclusion: The sensitive, rapid and selective UPLC-MS/MS method can be applied to the pharmacokinetic study of picroside I, II, III and IV in rats.

Keywords: Picroside, pharmacokinetics, bioavailability, rat, UPLC-MS/MS, plasma.

[1]
Li, P.; Matsunaga, K.; Ohizumi, Y. Nerve growth factor-potentiating compounds from Picrorhizae Rhizoma. Biol. Pharm. Bull., 2000, 23(7), 890-892.
[http://dx.doi.org/10.1248/bpb.23.890] [PMID: 10919373]
[2]
Wang, J.; Wang, L.; Lou, G.H.; Zeng, H.R.; Hu, J.; Huang, Q.W.; Peng, W.; Yang, X.B. Coptidis Rhizoma: a comprehensive review of its traditional uses, botany, phytochemistry, pharmacology and toxicology. Pharm. Biol., 2019, 57(1), 193-225.
[http://dx.doi.org/10.1080/13880209.2019.1577466] [PMID: 30963783]
[3]
Ma, H.; He, K.; Zhu, J.; Li, X.; Ye, X. The anti-hyperglycemia effects of Rhizoma Coptidis alkaloids: A systematic review of modern pharmacological studies of the traditional herbal medicine. Fitoterapia, 2019, 134, 210-220.
[http://dx.doi.org/10.1016/j.fitote.2019.03.003] [PMID: 30836124]
[4]
Han, L.; Wang, R.; Zhang, X.; Yu, X.; Zhou, L.; Song, T.; Deng, X.; Zhang, Y.; Zhang, L.; Bai, C. Advances in processing and quality control of traditional chinese medicine Coptidis rhizoma (Huanglian): A review. J. AOAC Int., 2019, 102(3), 699-707.
[http://dx.doi.org/10.5740/jaoacint.18-0303] [PMID: 30388971]
[5]
Wang, J.; Ran, Q.; Zeng, H.R.; Wang, L.; Hu, C.J.; Huang, Q.W. Cellular stress response mechanisms of Rhizoma coptidis: a systematic review. Chin. Med., 2018, 13, 27.
[http://dx.doi.org/10.1186/s13020-018-0184-y] [PMID: 29930696]
[6]
Dwivedi, A.K.; Kulkarni, D.; Singh, S. Sensitive high-performance liquid chromatographic assay method for the determination of picroside I in plasma. J. Chromatogr. B Biomed. Sci. Appl., 1997, 698(1-2), 317-320.
[http://dx.doi.org/10.1016/S0378-4347(97)00272-7] [PMID: 9367223]
[7]
Lv, J.; Pan, L.; Ye, Y.; Zhou, Y. A sensitive and selective RP-HPLC method for simultaneous determination of picroside-I and picroside-II in rat plasma and its application in pharmacokinetics studies. J. Sep. Sci., 2007, 30(15), 2466-2472.
[http://dx.doi.org/10.1002/jssc.200700139] [PMID: 17628865]
[8]
Yang, F.C.; Yang, S.L.; Xu, L.Z. Determination of picroside II in dog plasma by HPLC and its application in a pharmacokinetics study. Biomed. Chromatogr., 2005, 19(4), 279-284.
[http://dx.doi.org/10.1002/bmc.453] [PMID: 15651097]
[9]
Vipul, K.; Nitin, M.; Gupta, R.C. A sensitive and selective LC-MS-MS method for simultaneous determination of picroside-I and kutkoside (active principles of herbal preparation picroliv) using solid phase extraction in rabbit plasma: application to pharmacokinetic study. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2005, 820(2), 221-227.
[http://dx.doi.org/10.1016/j.jchromb.2005.03.032] [PMID: 15899375]
[10]
Cai, J.; Yang, X.; Lin, C. The effect China Bayberry on Cytochrome P450 in Rats by Cocktail Method. Lat. Am. J. Pharm., 2013, 32, 1452-1456.
[11]
Lin, C.; Cai, J.; Yang, X. Liquid chromatography-mass spectrometry determination of trigonelline in rat plasma and its application to a pharmacokinetic study. Lat. Am. J. Pharm., 2014, 33, 848-851.
[12]
Lin, C.; Chen, M.; Cai, J.; Lai, M.; Zheng, Y.; Yang, X. Determination of Emodin, Chrysophanol, and Physcion by HPLC in the Chinese Medicine Rumex japonicus Houtt. Lat. Am. J. Pharm., 2012, 31, 909-913.
[13]
Zheng, Y.; Wang, Y.; Zhang, L.; Zhang, Q.; Yang, X.; Lin, G. Pharmacokinetic interaction of entinostat and erlotinib in rat plasma following single and co-oral administration in rats. Lat. Am. J. Pharm., 2014, 33, 1225-1228.
[14]
Chen, J.; Bao, X.; Lin, C.; Zhou, G. Pharmacokinetics of rosmarinic acid in rats and tissue distribution in mice. Lat. Am. J. Pharm., 2019, 38, 985-990.
[15]
Wu, Q.; Wang, C.; Lin, Y.; Yang, S.; Wu, Y.; Fan, L.; Gu, K.; Hu, Y.; Wen, C.; Lin, C. Determination and validation of imperialine in rat plasma by ultra-performance liquid-chromatography with tandem mass spectrometry. Lat. Am. J. Pharm., 2015, 34, 1836-1841.
[16]
Wui, S.; Chen, B.; Zhang, J.; Wang, S.; Chen, M.; Wang, Q.; Wu, X.; Wen, C.; Zhou, Y.; Lin, C.; Zheng, H. Pharmacokinetic study of benzoylpaeoniflorin in rat plasma by UPLC-MS/MS. Lat. Am. J. Pharm., 2017, 36, 103-108.
[17]
Ye, W.; Lin, C.; Lin, G.; Chen, R.; Sun, W.; Wang, S.; Wang, X.; Zhou, Y. Tissue distribution of engeletin in mice by UPLC-MS/MS. Curr. Pharm. Anal., 2019, 15, 604-611.
[http://dx.doi.org/10.2174/1573412914666180501114659]
[18]
Chen, M.; Chen, Y.; Wang, X.; Zhou, Y. Quantitative determination of talatisamine and its pharmacokinetics and bioavailability in mouse plasma by UPLC-MS/MS. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2019, 1124, 180-187.
[http://dx.doi.org/10.1016/j.jchromb.2019.06.015] [PMID: 31207562]
[19]
Chen, R.; Lu, M.; Tu, X.; Sun, W.; Ye, W.; Ma, J.; Wen, C.; Wang, X.; Geng, P. Pharmacokinetics of panasenoside in rats and tissue distribution in mice by ultra-performance liquid chromatography tandem mass spectrometry. Acta Chromatogr., 2019, 31, 146-150.
[http://dx.doi.org/10.1556/1326.2018.00415]
[20]
Han, A.; Lin, G.; Cai, J.; Wu, Q.; Geng, P.; Ma, J.; Wang, X.; Lin, C. Pharmacokinetic study on hirsutine and hirsuteine in rats using UPLC-MS/MS. Acta Chromatogr., 2019, 31, 99-104.
[http://dx.doi.org/10.1556/1326.2017.00365]
[21]
Li, J.; Jin, Y.; Fu, H.; Huang, Y.; Wang, X.; Zhou, Y. Pharmacokinetics and bioavailability of gelsenicine in mice by UPLC-MS/MS. Biomed. Chromatogr., 2019, 33(3), e4418
[http://dx.doi.org/10.1002/bmc.4418] [PMID: 30367478]
[22]
Li, T.; Ye, W.; Huang, B.; Lu, X.; Chen, X.; Lin, Y.; Wen, C.; Wang, X. Determination and pharmacokinetic study of echinatin by UPLC-MS/MS in rat plasma. J. Pharm. Biomed. Anal., 2019, 168, 133-137.
[http://dx.doi.org/10.1016/j.jpba.2019.02.023] [PMID: 30807917]
[23]
Ren, K.; Jin, Y.; Wang, X.; Wei, Z. Pharmacokinetics of Isorhynchophylline in rat plasma after intravenous administration. Lat. Am. J. Pharm., 2019, 38, 991-995.
[24]
Song, H.; Huang, Y.; Zhu, D.; Tong, S.; Zhang, M.; Wang, X.; Bao, X. Pharmacokinetic study of deltaline in mouse blood based on UPLC-MS/MS. Curr. Pharm. Anal., 2019, 15, 194-199.
[http://dx.doi.org/10.2174/1573412914666181011124515]
[25]
Wang, S.; Zhang, Z.; Yu, Z.; Han, C.; Wang, X. Pharmacokinetic study of delavinone in mice after intravenous and oral administration by UPLC-MS/MS. BioMed Res. Int., 2019, 2019, 3163218
[http://dx.doi.org/10.1155/2019/3163218] [PMID: 31016188]
[26]
Wen, C.; Lin, F.; Huang, B.; Zhang, Z.; Wang, X.; Ma, J.; Lin, G.; Chen, H.; Hu, L. Metabolomics analysis in acute paraquat poisoning patients based on UPLC-Q-TOF-MS and machine learning approach. Chem. Res. Toxicol., 2019, 32(4), 629-637.
[http://dx.doi.org/10.1021/acs.chemrestox.8b00328] [PMID: 30807114]
[27]
Ye, W.; Sun, W.; Chen, R.; Wang, Z.; Cui, X.; Zhang, H.; Qian, S.; Zheng, Q.; Zhou, Y.; Wan, J.; Xu, J.; Wang, X.; Zhou, Y. Pharmacokinetics in rat plasma and tissue distribution in mice of galangin determined by UHPLC-MS/MS. Acta Chromatogr., 2019, 31, 120-125.
[http://dx.doi.org/10.1556/1326.2017.00389]
[28]
Zhou, Y.; Chen, B.; Chen, J.; Dong, Y.; Wang, S.; Wen, C.; Wang, X.; Yu, X. Determination and pharmacokinetic study of jaceosidin in rat plasma by UPLC-MS/MS. Acta Chromatogr., 2018, 30, 131-135.
[http://dx.doi.org/10.1556/1326.2017.00104]
[29]
Chen, L.; Weng, Q.; Li, F.; Liu, J.; Zhang, X.; Zhou, Y. Pharmacokinetics and bioavailability study of tubeimoside I in ICR mice by UPLC-MS/MS. J. Anal. Methods Chem., 2018, 2018, 9074893
[http://dx.doi.org/10.1155/2018/9074893] [PMID: 30116651]
[30]
Chen, L.; Weng, Q.; Ma, J. A new UPLC-MS/MS method validated for quantification of jervine in rat plasma and the study of its pharmacokinetics in rats. J. Anal. Methods Chem., 2019, 2019, 5163625
[http://dx.doi.org/10.1155/2019/5163625] [PMID: 30956840]
[31]
Lin, Y.; Pan, P.; Chen, L.; Yang, J.; Chen, D.; Wang, S.; Xiong, J. Simultaneous determination of doxofylline and its metabolite theophyl-line in rat plasma by ultra performance liquid chromatography. Curr. Pharm. Anal., 2016, 12, 296-299.
[http://dx.doi.org/10.2174/1573412912666151207190458]
[32]
Xiong, J.; Ye, H.; Lin, Y.; Yang, J.; Chen, D.; Yu, D.; Chen, L. Determining concentrations of icotinib in plasma of rat by uplc method with ultraviolet detection: applications for pharmacokinetic studies. Curr. Pharm. Anal., 2017, 13, 340-344.
[http://dx.doi.org/10.2174/1573412912666160815115244]


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 16
ISSUE: 4
Year: 2020
Page: [438 - 445]
Pages: 8
DOI: 10.2174/1573412916666191022161501
Price: $65

Article Metrics

PDF: 19
HTML: 2