Background: Formulations containing two or more than two herbs are called polyherbal
formulation. Single herb formulation has been well established due to its active phytoconstituents,
which are usually present in minute amount and sometimes these are insufficient to achieve the desired
therapeutic effects. The popularity of polyherbal formulation is due to its high effectiveness in a vast
number of diseases.
Methods: In this study, poly-herbal formulations were prepared by using different solvent extracts with
the help of the hot extraction process through Soxhlet apparatus. The poly-herbal formulations were
prepared by using traditional herbal plants namely, Bacopa monereri (BM), Embelica officinalis (EO),
Mucuna pruriens (MP), & Withania somnifera (WS). Different combinations of PHFs (PHF1-5) were
prepared by using the extracts in different ratios. The prepared PHFs were optimized for antioxidant
potential by the DPPH assay. Eighteen Swiss albino mice (30-50 g) were grouped into Control,
MPTP(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) and MPTP+PHF1 (6 mice in each group). Experimental
mice were given PHF1 (50 mg/kg body weight by intraperitoneal route). MPTP(1-methyl 4-
phenyl 1,2,3,6-tetrahydropyridine) treatment was given orally for 2 weeks with prior use of 20 mg/kg
body weight for 2 weeks.
Results: After the treatment period, neurobehavioral study and evaluation of neuroprotective effects
were done according to biochemical parameters.
Discussion: In the above study, it was found that PHF1 has upgraded the dopaminic neurons in the
brain. A significant diminution was found in SLA activity in MPTP treated mice as compared to the
control group which was restored by MPTP+PHF1 treated group as compared to MPTP treated group.
Conclusion: The result concluded that PHF1 enhanced the dopaminic neurons in the substantia nigra
region of the brain. Hence, this poly-herbal formulation gives a better and novel treatment base for future
drug discoveries in the case of Parkinson’s Disease (PD).