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Current Drug Metabolism

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ISSN (Print): 1389-2002
ISSN (Online): 1875-5453

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Research Article

Expression Levels of MicroRNA-125b in Serum Exosomes of Patients with Asthma of Different Severity and its Diagnostic Significance

Author(s): Meizhen Zhao*, Li Juanjuan, Fan Weijia, Xie Jing, Huang Qiuhua, Zhang Hua, Liao Fuhe and Peng Hao

Volume 20, Issue 10, 2019

Page: [781 - 784] Pages: 4

DOI: 10.2174/1389200220666191021100001

Price: $65

Abstract

Background: This study aimed to investigate the expression levels of microRNA (miRNA)-125b in serum exosomes and its diagnostic efficacy for asthma severity.

Methods: The study included 80 patients with untreated asthma and 80 healthy volunteers. The patients were divided into 4 groups according to disease severity: 20 with the intermittent state, 20 with the mildly persistent state, 20 with the moderately persistent state, and 20 with the severely persistent state. The expression levels of miRNA-125b in serum exosomes of each group were detected using a quantitative polymerase chain reaction and compared. The Spearman correlation analysis was used to study the correlation between the expression levels of miRNA-125b in serum exosomes and asthma severity. The diagnostic efficacy of the expression levels of miRNA-125b in exosomes for asthma severity was evaluated using the Receiver Operating Characteristic (ROC) curve.

Results: The expression levels of miRNA-125b in serum exosomes of patients with intermittent, mildly persistent, moderately persistent, and severely persistent asthma were all higher than those in the healthy control group, with statistically significant differences. The expression levels of miRNA-125b were also statistically significantly different among patients in each group. The Spearman correlation analysis showed a positive correlation of the relative expression of miRNA-125b in serum exosomes with asthma severity. The area under the ROC curve of the diagnostic efficacy of miRNA-125b in serum exosomes for patients with intermittent, mildly, moderately, and severely persistent asthma was 0.7770, 0.8573, 0.9111, and 0.9995, respectively.

Conclusion: The expression levels of miRNA-125b in serum exosomes had a high diagnostic efficacy and might serve as a noninvasive diagnostic marker for asthma severity.

Keywords: Asthma, exosomes, marker, microRNA-125b, DNMT1, lung.

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[1]
Lai, C.K.; Ko, F.W.; Bhome, A.; De Guia, T.S.; Wong, G.W.; Zainudin, B.M.; Nang, A.N.; Boonsawat, W.; Cho, S.H.; Gunasekera, K.D.; Hong, J.G.; Hsu, J.Y.; Viet, N.N.; Yunus, F.; Mukhopadhyay, A. Relationship between asthma control status, the Asthma Control Test™ and urgent health-care utilization in Asia. Respirology, 2011, 16, 688-697.
[http://dx.doi.org/10.1111/j.1440-1843.2011.01954.x] [PMID: 21362102]
[2]
Nan, S.; Wu, C.G.; Lin, J.T.; Yuan, Y.D.; Xin, Z.; Yin, K.S. In: Investigation on the status quo and disease cognition of asthma patients in urban areas of China, Proceedings of the sixth national asthma academic conference of Chinese medical association and the second conference of China asthma federation, China. 2008.
[3]
Asthma group. Respiratory medicine branch. Chinese medical association. Guidelines for prevention and treatment of bronchial asthma. Zhonghua Jie He He Hu Xi Za Zhi, 2008, 31, 177-185.
[4]
Liard, R.; Leynaert, B.; Zureik, M.; Beguin, F.X.; Neukirch, F. Using global initiative for asthma guidelines to assess asthma severity in populations. Eur. Respir. J., 2000, 16, 615-620.
[http://dx.doi.org/10.1034/j.1399-3003.2000.16d08.x] [PMID: 11106201]
[5]
Doldán, X.; Fagúndez, P.; Cayota, A.; Laíz, J.; Tosar, J.P. Electrochemical sandwich immunosensor for determination of exosomes based on surface markermediated signal amplification. J. Anal. Chem., 2016, 88, 10466-10473.
[http://dx.doi.org/10.1021/acs.analchem.6b02421] [PMID: 27734678]
[6]
Harris, D.A.; Patel, S.H.; Gucek, M.; Hendrix, A.; Westbroek, W.; Taraska, J.W. Exosomes released from breast cancer carcinomas stimulate cell movement. PLoS One, 2015, 10(3)e117495
[http://dx.doi.org/doi:10.1371/journal.pone.0117495] [PMID: 25798887]
[7]
Bai, F.X.; Jue, P. Research status of exosome and lung diseases. Int. J. Respir., 2015, 5, 629-632.
[8]
Munitz, A.; Karo-Atar, D.; Foster, P.S. Asthma diagnosis: microRNAs to the rescue. J. Allergy Clin. Immunol., 2016, 137, 1447-1448.
[http://dx.doi.org/10.1016/j.jaci.2016.02.013] [PMID: 27155036]
[9]
Rożalski, M.; Rudnicka, L.; Samochocki, Z. MiRNA in atopic dermatitis. Postepy Dermatol. Alergol., 2016, 33, 157-162.
[http://dx.doi.org/doi:10.5114/ada.2016.60606] [PMID: 27512348]
[10]
Levänen, B.; Bhakta, N.R.; Paredes, P.T.; Barbeau, R.; Hiltbrunner, S.; Pollack, J.L. Sköld, C.M.; Svartengren, M; Grunewald, J.; Gabrielsson, S.; Eklund, A; Larsson, B.-M.; Woodruff, P.G.; Erle, D.J.; Wheelock, A.M. Altered microRNA profliles in bronehoalveolar lavage fluid exosomes in asthmatic patients. J. Allergy Clin. Immunol., 2013, 131, 894-903.
[http://dx.doi.org/doi:10.1016/j.jaci.2012.11.039] [PMID: 23333113]
[11]
Yong, L.; Gao, C.B.; Sheng, W.W.; Guan, H.L. Expression and clinical significance of mirna-125b and mirna-133b in plasma of children with bronchial asthma. J. Modern Lab. Med., 2017, 32, 59-62.
[12]
Sánchez-Vidaurre, S.; Eldh, M.; Larssen, P.; Daham, K.; Martinez-Bravo, M-J.; Dahlén, S-E.; Dahlén, B.; Van Hage, M.; Gabrielsson, S. RNA-containing exosomes in induced sputum of asthmatic patients. J. Allergy Clin. Immunol., 2017, 140(5), 1459-1461.
[http://dx.doi.org/10.1016/j.jaci.2017.05.035] [PMID: 28629752]

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