Title:Simultaneous Assessment of MicroRNAs 126 and 192 in Diabetic Nephropathy Patients and the Relation of these MicroRNAs with Urinary Albumin
VOLUME: 20 ISSUE: 5
Author(s):Safaa I. Tayel*, Amany A. Saleh, Sally M. El-Hefnawy, Khaled MA. Elzorkany, Ghada E. Elgarawany and Rasha I. Noreldin
Affiliation:Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Menoufia University, Internal Medicine Department, Faculty of Medicine, Menoufia University, Physiology Department, Faculty of Medicine, Menoufia University, Clinical Pathology Department, Faculty of Medicine, Menoufia University
Keywords:Diabetic nephropathy, microalbuminuria, MicroRNA, renal functions, urinary albumin, nephropathy.
Abstract:Background and Objective: Diabetic nephropathy (DN) is a major
determinant of end-stage renal disease (ESRD). Altered microRNA levels lead to
serious chronic diseases, such as diabetes. We aimed to measure the expression levels
of two microRNAs, microRNA126 and 192 in DN and investigate their connection with
albuminuria levels.
Methods: This study included 229 subjects (134 DN patients and 95 controls). Serum
lipid profiles, glucose levels, glycated haemoglobin (HbA1c) levels, and renal functions
were assayed. The microRNA126 and microRNA192 expression levels were determined
by real-time PCR.
Results: Patients with DN had higher weights, BMI values, glucose levels (P<0.001),
HbA1c levels (P<0.001), urinary albumin-creatinine ratio (ACR) values (P<0.001), urea
levels (P=0.002), and creatinine levels (P=0.004) and lower expression levels of both
microRNA192 (P<0.001) and microRNA126 (P<0.001) than controls. MicroRNA126
expression was positively correlated with age, estimated glomerular filtration rate
(eGFR) and microRNA192 expression but negatively correlated with blood sugar,
HbA1c, urea, creatinine and ACR. MicroRNA192 had higher sensitivity (91%), specificity
(94%), and area under the curve (AUC) (0.967) values than microRNA126 (sensitivity,
90%; specificity, 68%; AUC, 0.897) and thus can precisely diagnose DN.
Conclusion: Both MicroRNA126 and microRNA192 expression were obviously
associated with DN and might determine the progression of the disease owing to
prominent relation with macroalbuminuria.
