Background: Pseudouridine (Ψ) is the most abundant RNA modification and has
important functions in a series of biological and cellular processes. Although experimental
techniques have made great contributions to identify Ψ sites, they are still labor-intensive and costineffective.
In the past few years, a series of computational approaches have been developed,
which provided rapid and efficient approaches to identify Ψ sites.
Results: To provide the readership with a clear landscape about the recent development in this
important area, in this review, we summarized and compared the representative computational
approaches developed for identifying Ψ sites. Moreover, future directions in computationally
identifying Ψ sites were discussed as well.
Conclusion: We anticipate that this review will provide novel insights into the researches on