Aim: This study aims to find out the components responsible for the antithrombotic activity
of Nelumbo nucifera.
Material and Methods: Petroleum ether, chloroform and hydroalcoholic extracts of whole plant of
Nelumbo nucifera (Lotus) were prepared and assessed for its thrombolytic, anti-platelet aggregation
activity and bleeding time. The extracts were further analyzed through HPTLC and GC-MS. Statistical
analysis was conducted through ANOVA trailed by Tukey’s multiple comparison test test.
Results: Hydroalcoholic extract showed the highest activity at the concentration of 400µg/ml in
thrombolytic assay (42.03 ± 5.76), anti-platelet aggregation assay (57.93 ± 1.68) and bleeding time
(70.17 ± 2.16) in comparison to clopodigrel (33.76 ± 3.43), aspirin (66.55 ± 1.86) and aspirin (93.85
± 2.75) at the concentration of 100 µg/ml respectively. 25 peaks were identified through GC-MS, out
of which, ferulic acid (14.2µ/g) and quercetin (5.4 µ/g) are active chemical compounds. HPTLC
showed different chromatograms in hydroalcoholic extracts like (1) chlorogenic, (2) quercetin, (3)
benzoic acid, (4) caffeic acid, (5) ferulic acid, (6) kaempferol, and (7) gallic acid.
Conclusion: Based on these findings, flavonoids present in hydroalcoholic extract may be developed
into a drug for clinical application for the treatment of thrombosis in patients.