Title:Interactions of Flavone and Steroid from A. subintegra as Potential Inhibitors for Porcine Pancreatic Lipase
VOLUME: 16 ISSUE: 6
Author(s):Mastura Ibrahim, Saripah Salbiah Syed Abdul Azziz*, Chee Fah Wong*, Yuhanis Mhd Bakri and Fauziah Abdullah
Affiliation:Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim, Perak, Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim, Perak, Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim, Perak, Department of Chemistry, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris, 35900 Tanjong Malim, Perak, Phytochemistry Programme, Natural Products Division, Forest Research Institute Malaysia, 52109 Kepong, Selangor
Keywords:Aquilaria subintegra, lipase, flavonoid, docking, competitive, YASARA.
Abstract:
Background: Obesity is one serious health condition that contributes to various chronic diseases.
The inhibition of pancreatic lipase is a promising treatment for obesity.
Objective: The present study was designed to investigate anti-porcine pancreatic lipase effect of isolated
compounds from Aquilaria subintegra and its mechanism.
Methods: Compounds were isolated with serial column chromatography and their structure were identified
using spectroscopic methods. Isolated compounds were tested for anti-lipase potential activity using
colorimetric assay. The prediction of energy binding between isolated compounds and enzyme was
described using YASARA software.
Results: Four compounds were successfully isolated from the bark of A. subintegra, namely, 5-
hydroxy-7,4’-dimethoxyflavone, luteolin-7,3’,4’-trimethyl ether, 5,3’-dihydroxy-7,4’-dimethoxyflavone
and β-sitosterol. The results indicated that all compounds displayed promising pancreatic lipase
inhibitory activity ranging between of 6% to 53% inhibition. Compound 5-hydroxy-7,4’-
dimethoxyflavone was a competitive inhibitor and decreases the enzyme catalysis. Meanwhile, β-
sitosterol was a non- competitive inhibitor since the latter was bind allosterically toward enzyme.
Conclusion: This finding is significant for further investigation of bioactive compounds from A. subintegra
on animal study.
