Background: Obesity is one serious health condition that contributes to various chronic diseases.
The inhibition of pancreatic lipase is a promising treatment for obesity.
Objective: The present study was designed to investigate anti-porcine pancreatic lipase effect of isolated
compounds from Aquilaria subintegra and its mechanism.
Methods: Compounds were isolated with serial column chromatography and their structure were identified
using spectroscopic methods. Isolated compounds were tested for anti-lipase potential activity using
colorimetric assay. The prediction of energy binding between isolated compounds and enzyme was
described using YASARA software.
Results: Four compounds were successfully isolated from the bark of A. subintegra, namely, 5-
hydroxy-7,4’-dimethoxyflavone, luteolin-7,3’,4’-trimethyl ether, 5,3’-dihydroxy-7,4’-dimethoxyflavone
and β-sitosterol. The results indicated that all compounds displayed promising pancreatic lipase
inhibitory activity ranging between of 6% to 53% inhibition. Compound 5-hydroxy-7,4’-
dimethoxyflavone was a competitive inhibitor and decreases the enzyme catalysis. Meanwhile, β-
sitosterol was a non- competitive inhibitor since the latter was bind allosterically toward enzyme.
Conclusion: This finding is significant for further investigation of bioactive compounds from A. subintegra
on animal study.