New chemical agents that could combat increasing antibiotic resistance are urgently
needed. In this mini-review, an old but highly relevant RNA sequence which is crucial for the continuation
of bacterial life-cycle is covered. Some of the most significant advances of the last decade
in sensing and targeting the bacterial rRNA A-site: a well-validated binding site of proverbially
known aminoglycoside antibiotics are described. Some of the major advances in direct sensing
of the bacterial decoding side (A-site) are described and also new fluorescent molecules that are
capable of detecting lead compounds through high-throughput assays by displacement of fluorescent
probe molecules are highlighted. Lastly, some of the recently discovered non-aminoglycoside
small molecule binders of bacterial rRNA A-site as a new class of molecules that could provide
future scaffolds and molecules for developing new antibacterial agents have been discussed.
Keywords: A-site, rRNA, FRET, Antimicrobial agents, Drug Discovery.
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