Lopinavir Loaded Spray Dried Liposomes with Penetration Enhancers for Cytotoxic Activity

Author(s): Mithun Maniyar*, Ashok Chandak, Chandrakant Kokare

Journal Name: Infectious Disorders - Drug Targets
Formerly Current Drug Targets - Infectious Disorders

Volume 20 , Issue 5 , 2020


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Abstract:

Objective: HIV protease inhibitors (HIV-PI) are the drugs utilized for the treatment of HIV. However, their effectiveness is limited due to lack of bioavailability and they need to be coadministered with another drug. In this study single lopinavir (LPV) loaded phospholipid vesicles were prepared by the spray-drying method. The LPV-loaded spray-dried powder (L-SDP) was transformed into vesicles and then entrapped in a cream base with peppermint and olive oil.

Method: It is an Attenuated Total Reflectance Fourier Transform Infrared (ATR-FTIR) membrane fluidity study that is used to predict oil’s effect on skin. The central composite design was used to optimize the L-SDP cream formulation. Ex-vivo drug release, skin deposition study, and cell proliferation assays were carried out using cancer cell lines of breast, lung, and skin melanoma. Analysis of DNA by flow cytometry on human breast cancer cell line MDA-MB-231 was carried out. The fluorescence microscopy, histopathological study, and in-vivo bioavailability studies were performed to measure the penetration and inertness of cream in animals.

Results: A membrane fluidity study revealed the effectiveness of oils as penetration enhancers. The L-SDP cream showed comparatively superior (%) drug deposition and permeability . Fluorescence images further confirm the penetration ability of the L-SDP cream which showed promising antiproliferative action on breast and lung cancer cells. The histopathological study demonstrates the inertness of cream while in-vivo bioavailability studies showed the many-fold increase in bioavailability of LPV.

Conclusions: The liposomal drug delivery system of LPV has the potential to expose skin to systemic circulation and is useful for treating cancer.

Keywords: Liposomes, membrane fluidity study, cell proliferation assay, flow cytometry, fluorescence microscopy, in-vivo bioavailability studies.

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VOLUME: 20
ISSUE: 5
Year: 2020
Page: [724 - 736]
Pages: 13
DOI: 10.2174/1871526519666191008112207
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