Background: Collagenase is a type of proteolytic enzyme that specifically hydrolyzes native
collagen with a three-dimensional helical structure. The structure and properties of collagenase
vary with different sources and types. In addition to the well-characterized Clostridium collagenase,
other collagenases are largely unknown. Various gene and protein databases have been widely used to
mine novel functional genes in the genome. Gene mining and sequence analysis are effective methods
for studying these enzymes.
Objective: The present study aimed to understand the molecular, structural, and functional characteristics
of collagenase from Bacillus cereus MH19 using a bioinformatics approach.
Methods: Based on the three-generation sequencing PacBio technique, Sequencing the Bacillus cereus
MH19 genome. Function annotation is completed by blasting genes with different databases. Collagenases
were investigated based on the physiochemical properties, phylogenetic relation, and domain
architecture. The 3D structure model of the selected collagenase has been constructed and verified by
Results: There were 5 collagenases in Bacillus cereus MH19 with a molecular weight distribution
ranging from 36-110 kDa. The analysis of evolutionary relationship between different collagenases
indicating that the BCC000504 and BCC003388 collagenase gene sequences are closer to the typical
collagenase genes ColG, ColA, and ColH, followed by BCC003615. The domain and function analysis
showed that the collagenases BCC000504 and BCC003388 were similar to the collagenases ColG,
ColA and ColH. BCC004271 was similar to BCC004272, and BCC003615 might be quite different
from other collagenases. The secondary structure of collagenase was analyzed. The SAVES evaluation
indicates that 3D structural modeling of the selected collagenase is acceptable.
Conclusion: This study provides an overview of the molecular, functional, and structural characteristics
of collagenase from Bacillus cereus MH19, which helps to understand the bacterial collagenase. The
characterization of the collagenase will certainly expand the application range of collagenase.