Background: Aspergillus terreus is a major etiological agent among fungal pathogens and
emerged as an opportunistic pathogen in immunocompromised patients.
Objective: To explore mycelial proteins to shed light on chemical and molecular details that contribute
to pathogenesis including intrinsic resistant to amphotericin B (AmB), and biomolecules associated
with biofilm formation.
Methods: The mycelial proteins of A. terreus cultured for 48h at 37°C have been analysed by using
nLC-ESI-MS/MS. Protein data analysis performed by Proteome Discoverer (V/2.2) against the
UniProt database. Also, Scanning Electron Microscopy (SEM) analysis of biofilm formation under optimum
conditions was carried out. qRT-PCR was carried out for genes encoding for functionally important
Results: A total of 389 proteins were detected at FDR 0.01. Gene ontology showed the abundance of
proteins from energy metabolism, cellular homeostasis, ribosome biogenesis, cell wall, and structural
components. We observed catalase, superoxide dismutase, thioredoxin, glutathione S-transferase involved
in redox homeostasis and heat shock proteins (Hsp90 and Hsp70). These proteins may provide
insight into the resistance mechanism of A. terreus against AmB. Additionally, SEM analysis of A.
terreus showed the formation of dense hyphal network covered with porous extracellular matrix
(ECM). Using SECRETOOL, 8 proteins were predicted as secretory. Three proteins, such as glucanase
Crf1/allergen (Asp F9), 1, 3-β-glucanosyltransferase and β-hexosaminidase were reported in biofilm
Conclusion: Our proteome data provided experimental evidence to the annotated set of proteins in A.
terreus that comprehend biochemical and cellular events involved in the establishment of mycelial
network and contributing to the pathogenesis. This work also provides resource for further molecular
studies in A. terreus.