Background: There is some experimental data on the effect exerted by some
steroid derivatives against ischemia/reperfusion injury; however, the molecular mechanism
is very confusing, perhaps this phenomenon could be due to the protocols used and/or differences
in the chemical structure of each one of the steroid derivatives.
Objectives: The aim of this study was to synthesize a new bis-steroid-methanocyclobutanaphthalene-
dione derivative using some tools chemical.
Methodology: The biological activity exerted by the bis-steroid-methanocyclobutanaphthalene-
dione derivative against ischemia/reperfusion injury was evaluated in an isolated
heart model using noradrenaline, milrinone, dobutamine, levosimendan, and Bay-K-
8644 as controls. In addition, other alternative experiments were carried out to evaluate the
biological activity induced by the bis-steroid-methanocyclobuta-naphthalene-dione derivative
against left ventricular pressure in the absence or presence of nifedipine.
Results: The results showed that 1) the bis-steroid-methanocyclobuta-naphthalene-dione
derivative significantly decreases the ischemia-reperfusion injury translated as a decrease
in the the infarct area in a similar manner to levosimendan drug; 2) both bis-steroidmethanocyclobuta-
naphthalene-dione and Bay-K-8644 increase the left ventricular pressure
and 3) the biological activity exerted by bis-steroid-methanocyclobuta-naphthalenedione
derivative against left ventricular pressure is inhibited by nifedipine.
Conclusion: In conclusion, the bis-steroid-methanocyclobuta-naphthalene-dione derivative
decreases the area of infarction and increases left ventricle pressure via calcium channels
activation; this phenomenon could constitute a new therapy for ischemia/reperfusion injury.