Background: Type 1 diabetes mellitus is characterized by the destruction of insulin-
producing Beta cells in the pancreas. Researchers hope that islet transplantation will
help to patients with insulin-dependent diabetes mellitus (IDDM). Oxidative stress is the
most important challenge that beta cells face to it after isolation, and mitochondrial dysfunction
is a crucial mediator in beta cells death. Hence, therapeutic approaches can shift to
antioxidants through the application of nanoparticles such as cerium and yttrium oxide nanoparticles
(Cer and Ytt Ox NPs) and nano-selenium (Nan Se).
Objective: This study evaluates the effects of Cer and Ytt Ox NPs and Nan Se on H2O2-
induced oxidative stress in pancreatic beta cells with focus on mitochondrial dysfunction
Methods: CRI-D2 beta-cell line were pretreated with Cer Ox NPs (200 µM) + Ytt Ox NPs
(0.5 µg/mL) for 3 days and/or Nan Se (0.01 µM) for 1 day. Then markers of oxidative
stress, mitochondrial dysfunction, insulin and glucagon secretion were measured.
Results: We reported a decrease in H2O2-induced reactive oxygen species (ROS) level and
glucagon secretion, and an increase in H2O2-reduced ATP/ADP ratio, MMP, as well as
UCP2 protein expression, and insulin secretion by pretreatment of CRI-D2 cells with Cer
and Ytt Ox NPs and/or Nan Se.
Conclusion: We found maximum protective effect with Cer and Ytt Ox NPs on CRI-D2
beta-cell line exposed by H2O2 for keeping beta cells alive until transplant whereas combination
of Cer and Ytt Ox NPs and Nan Se had very little protective effect in this condition.