Background and Objective: Bacopa monnieri, Evolvulus alsinoides, and Tinospora cordifolia
are well known herbal Ayurvedic medicinal plants with a memory enhancing property. The aim of
this study was HPTLC analysis for evaluating the effect of ethanolic extract of Bacopa monnieri
(BME), Evolvulus alsinoides (EAE) and Tinospora cordifolia (TCE) alone and in combination of equal
proportion (CEPs) against scopolamine-induced amnesic rats.
Methods: HPTLC analysis was done to evaluate the presence of phytoconstituents in these plants. Morris
water maze and passive avoidance tests were also used to evaluate the effect on memory function.
All pretreatment with BME, EAE, TCE, and CEPs was done at 200 mg/kg. The observation was done
on the basis of the effect on mean latency time (in seconds) in the Morris water maze test and latency to
reach shock free zone (SFZ), mistakes in 15 minutes on the Passive-avoidance test.
Results: It was observed that after HPTLC analysis, Bacoside A (Rf value; 0.82) found in BME, flavonoid
glycoside (Rf value; 0.31) found in EAE and unknown compounds (Rf value; 0.11, 0.39) observed
in TCE. BME, EAE, TCE and their combinations of equal proportion [CEP-1 (BME+EAE),
CEP-2 (BME+TCE), CEP-3 (EAE+TCE) and CEP-4 (BME+EAE+TCE)] produced significant effects
against scopolamine-induced amnesia in rats.
Conclusion: These findings suggested that the content of extracts of these compounds has an antioxidant
property in the brain. Also, BME, EAE, and TCE in the CEPs provide a synergistic effect on behavioral