Background: Antimicrobial potential of silver nanoparticles synthesised by using
various biological sources was already been reported by many researchers. The green synthesis of
silver nanoparticles using microbial sources has been proved to be more effective.
Methods: In this study, anti-dermatophytic silver nanoparticles were synthesised by using pyrrolo
metabolites producing actinomycetes as a green catalyst. Different characterization methods such
as UV-Visible, XRD, and AFM were used to identify the physiochemical characteristics of synthesised
Results: The synthesised nanoparticles showed λ-max at 427 nm and 402 nm, respectively. The
XRD analysis based on the JCPDS database identified the two synthesized nanoparticles as silver
oxide nanoparticles (AgO NPs) and silver peroxide nanoparticles (Ag2O3 NPs). The size of these
nanoparticles was found to be in the range of 40-44 nm (AgO NPs) and 23-25 nm (Ag2O3 NPs),
respectively. The synthesized nanoparticles demonstrated significant anti-fungal activity against
dermatophytic fungi Trichophyton mentagrophyes with the zone of inhibition of 38 mm by AgO
NP and 17 mm by Ag2O3 NPs.
Conclusion: Screening of marine actinomycetes LG003 and LG005 revealed the presence of
pyrrolo derivatives as the major metabolites, suggesting that these pyrrolo derivatives could be responsible
for synthesis and stabilization of AgO and Ag2O3NPs. Among the synthesized NPs, the
AgO NPs showed great potential as an anti-dermatophytic agent. This study provides further research
opportunities for AgONPs as anti-fungal agents.