The hyphenation of Ultra-Performance Liquid performance (UPLC) with mass spectrometry
(MS) has emerged as a powerful tool in analytical research due to its advanced sensitivity,
resolution and speed. Its advanced instrumentation, specialized columns, separation at ultra-high
pressure and sophisticated software are the distinguishing features as compared to the traditional
separating techniques. It has a wide range of applications in various fields such as analysis of food
stuffs, drug metabolites, beverages, toxicology, soil samples and micronutrient analysis. In the present
compilation, authors have highlighted the applicability of UPLC-MS in the analysis of food stuffs and
drug metabolites along with the various optimized analytical conditions and obtained results.