Infectious diseases have caused historical pandemics in the world. Three strategies, including
sanitation programs, antimicrobial drugs, and vaccines are considered for the prevention and treatment
of infectious diseases. Today, some infectious diseases cause millions of mortalities universally.
Due to the emergence of antibiotic-resistant pathogens, as well as some limitations of traditional vaccines,
focusing on novel strategies is essential. Multi-Epitope Vaccines (MEVs), as a novel strategy,
have been designed based on immunoinformatics methods; epitope prediction by authentic servers,
attachment of epitopes using proper linkers, physicochemical, immunological and structural evaluation
by bioinformatics tools that are basic stages in MEVs designing. Advantages such as cost-effective,
high safety, less time consumption in designing, the application of natural adjuvants, and satisfactory
preclinical evaluation outstand MEVs than other types of vaccines. Therefore, MEVs are promising
vaccines against resistant diseases such as lower respiratory infection and diarrhea.