Efficacy and Safety Curcuma zadoaria L. to Inactivate the Hydatid Cyst Protoscoleces

Author(s): Hossein Mahmoudvand, Mahbobeh Pakravanan, Farnaz Kheirandish, Sareh Jahanbakhsh, Maryam Sepahvand, Massumeh Niazi, Abdolreza Rouientan, Mohammad R. Aflatoonian*

Journal Name: Current Clinical Pharmacology
Continued as Current Reviews in Clinical and Experimental Pharmacology

Volume 15 , Issue 1 , 2020


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Abstract:

Background: The present work aimed to evaluate the chemical composition of Curcuma zadoaria essential oil and to investigate its efficacy and safety against hydatid cyst protoscoleces.

Methods: Collected protoscoleces from liver fertile hydatid cysts of infected sheep were exposed to different concentrations of the essential oil (75, 150, 300 μl/mL) for 5-30 min in vitro and ex vivo. Then, by using the eosin exclusion assay, the viability of protoscoleces was studied. In the next step, 24 male NMRI mice were examined to assess the toxicity of C. zadoaria essential oil by measuring the biochemical and hematological parameters.

Results: Based on the obtained results, the LD50 value of intraperitoneal injection of the C. zadoaria essential oil was 1.76 mL/kg of body weight and the maximum non-fatal dose was 0.96 mL/kg of body weight. C. zadoaria essential oil had a strong proto scolicidal activity in vitro so that at the 300 and 150 μl/ml entirely eliminates the parasite after 5 and 10 minutes; whereas, weak proto scolicidal activity was observed at lower doses. Ex vivo assay, no similar effect with in vitro was observed, therefore, more time is required to show a potent proto scolicidal activity. C. zadoaria essential oil at the concentrations of 300 and 150 μl/mL after an exposure time of 7 and 12 min, killed 100% of protoscoleces within the hydatid cyst, respectively. After intraperitoneal injection of the C. zadoaria essential oil for 2 weeks, no significant difference (p > 0.05) was observed in the clinical chemistry and hematologic parameters at the doses of 0.15, 0.3, 0.6 mL/kg.

Conclusion: The obtained results in vitro and ex vivo exhibited that C. zadoaria essential oil had a favorable proto scolicidal activity on hydatid cyst protoscoleces. However, more supplementary works are required to verify these findings by assessing clinical subjects.

Keywords: GC/MS, cystic echinococcosis, Echinococcus granulosus, protoscoleces, white turmeric, toxicity.

[1]
World Health Organization (WHO) informal working group on echinococcosis. Bull World Health Organ 1996; 74: 231-42.
[2]
Brunetti E, Kern P, Vuitton DA. Writing Panel for the WHO-IWGE. Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop 2010; 114(1): 1-16.
[http://dx.doi.org/10.1016/j.actatropica.2009.11.001] [PMID: 19931502]
[3]
Junghanss T, da Silva AM, Horton J, Chiodini PL, Brunetti E. Clinical management of cystic echinococcosis: state of the art, problems, and perspectives. Am J Trop Med Hyg 2008; 79(3): 301-11.
[http://dx.doi.org/10.4269/ajtmh.2008.79.301] [PMID: 18784219]
[4]
McManus DP, Zhang W, Li J, Bartley PB. Echinococcosis. Lancet 2003; 362(9392): 1295-304.
[http://dx.doi.org/10.1016/S0140-6736(03)14573-4] [PMID: 14575976]
[5]
Mahmoudvand H, Mirbadie SR, Ghasemi Kia M, Badparva E, Shamsadini Lori S, Fasihi Harandi M. Efficacy of Pistacia khinjuk fruits on viability of hydatid cyst protoscoleces and its acute toxicity in mice model. Iran J Parasitol 2016; 11(3): 383-8.
[PMID: 28127345]
[6]
Rajabi MA. Fatal reactions and methaemoglobinaemia after silver nitrate irrigation of hydatid cyst. Surg Pract 2009; 13: 2-7.
[http://dx.doi.org/10.1111/j.1744-1633.2008.00427.x]
[7]
Sahin M, Eryilmaz R, Bulbuloglu E. The effect of scolicidal agents on liver and biliary tree (experimental study). J Invest Surg 2004; 17(6): 323-6.
[http://dx.doi.org/10.1080/08941930490524363] [PMID: 15764499]
[8]
Kohansal MH, Nourian A, Rahimi MT, Daryani A, Spotin A, Ahmadpour E. Natural products applied against hydatid cyst protoscolices: A review of past to present. Acta Trop 2017; 176: 385-94.
[http://dx.doi.org/10.1016/j.actatropica.2017.09.013] [PMID: 28935552]
[9]
Wilson B, Abraham G, Manju VS, et al. Antimicrobial activity of Curcuma zedoaria and Curcuma malabarica tubers. J Ethnopharmacol 2005; 99(1): 147-51.
[http://dx.doi.org/10.1016/j.jep.2005.02.004] [PMID: 15848035]
[10]
Lobo R, Prabhu KS, Shirwaikar A, Shirwaikar A. Curcuma zedoaria Rosc. (white turmeric): a review of its chemical, pharmacological and ethnomedicinal properties. J Pharm Pharmacol 2009; 61(1): 13-21.
[http://dx.doi.org/10.1211/jpp.61.01.0003] [PMID: 19126292]
[11]
Saedi Dezaki E, Mahmoudvand H, Sharififar F, Fallahi S, Monzote L, Ezatkhah F. Chemical composition along with anti-leishmanial and cytotoxic activity of Zataria multiflora. Pharm Biol 2015; 8: 1-7.
[PMID: 26449681]
[12]
Tavakoli Kareshk A, Keyhani A, Mahmoudvand H, et al. Efficacy of the Bunium Persicum (Boiss) essential oil against acute toxoplasmosis in mice model. Iran J Parasitol 2015; 10(4): 625-31.
[PMID: 26811730]
[13]
Adams RP. Identification of essential oil components by gas chromatography/mass spectroscopy Illinois, USA. Carol Stream, IL: Allured Publishing Corporation 2004.
[14]
Niazi M, Saki M, Sepahvand M, Jahanbakhsh S, Khatami M, Beyranvand M. In vitro and ex vivo scolicidal effects of Olea europaea L. to inactivate the protoscolecs during hydatid cyst surgery. Ann Med Surg (Lond) 2019; 42(42): 7-10.
[http://dx.doi.org/10.1016/j.amsu.2019.04.006] [PMID: 31193397]
[15]
Monteiro DU, Azevedo MI, Weiblen C, et al. In vitro and ex vivo activity of Melaleuca alternifolia against protoscoleces of Echinococcus ortleppi. Parasitology 2017; 144(2): 214-9.
[http://dx.doi.org/10.1017/S0031182016001621] [PMID: 27762176]
[16]
Shakibaie M, Shahverdi AR, Faramarzi MA, Hassanzadeh GR, Rahimi HR, Sabzevari O. Acute and subacute toxicity of novel biogenic selenium nanoparticles in mice. Pharm Biol 2013; 51(1): 58-63.
[http://dx.doi.org/10.3109/13880209.2012.710241] [PMID: 23035822]
[17]
Posadzki P, Watson L, Ernst E. Herb-drug interactions: an overview of systematic reviews. Br J Clin Pharmacol 2013; 75(3): 603-18.
[http://dx.doi.org/10.1111/j.1365-2125.2012.04350.x] [PMID: 22670731]
[18]
Kabak B, Dobson AD. Mycotoxins in spices and herbs-An update. Crit Rev Food Sci Nutr 2017; 57(1): 18-34.
[http://dx.doi.org/10.1080/10408398.2013.772891] [PMID: 26528824]
[19]
Badawy H, Brunellière J, Veryaskina M, et al. Assessing the antimicrobial activity of polyisoprene based surfaces. Int J Mol Sci 2015; 16(3): 4392-415.
[http://dx.doi.org/10.3390/ijms16034392]
[20]
Migoń D, Neubauer D, Kamysz W. Hydrocarbon stapled antimicrobial peptides. Protein J 2018; 37(1): 2-12.
[http://dx.doi.org/10.1007/s10930-018-9755-0] [PMID: 29330644]
[21]
Sikkema J, de Bont JA, Poolman B. Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev 1995; 59(2): 201-22.
[http://dx.doi.org/10.1128/MMBR.59.2.201-222.1995] [PMID: 7603409]
[22]
Cristani M, D’Arrigo M, Mandalari G, et al. Interaction of four monoterpenes contained in essential oils with model membranes: implications for their antibacterial activity. J Agric Food Chem 2007; 55(15): 6300-8.
[http://dx.doi.org/10.1021/jf070094x] [PMID: 17602646]
[23]
Ismail A, Lamia H, Mohsen H, et al. Chemical composition and antifungal activity of three anacardiaceae species grown in tunisia. Sci Int 2013; 1: 148-54.
[http://dx.doi.org/10.5567/sciintl.2013.148.154]
[24]
Lai EY, Chyau CC, Mau JL, et al. Antimicrobial activity and cytotoxicity of the essential oil of Curcuma zedoaria. Am J Chin Med 2004; 32(2): 281-90.
[http://dx.doi.org/10.1142/S0192415X0400193X] [PMID: 15315265]
[25]
Loomis TA. Essential of Toxicology. Philadelphia: Lea and Febige 1968.


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Article Details

VOLUME: 15
ISSUE: 1
Year: 2020
Published on: 04 May, 2020
Page: [64 - 71]
Pages: 8
DOI: 10.2174/1574884714666190918155147

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